Development of the olfactory epithelium and Nasal Glands in TMEM16A-/-and TMEM16A+/ +Mice

Devendra Kumar Maurya, Tiago Henriques, Monica Marini, Nicoletta Pedemonte, Luis J V Galietta, Jason R. Rock, Brian D. Harfe, Anna Menini Menini

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

TMEM16A/ANO1 is a calcium-activated chloride channel expressed in several types of epithelia and involved in various physiological processes, including proliferation and development. During mouse embryonic development, the expression of TMEM16A in the olfactory epithelium is dynamic. TMEM16A is expressed at the apical surface of the entire olfactory epithelium at embryonic day E12.5 while from E16.5 its expression is restricted to a region near the transition zone with the respiratory epithelium. To investigate whether TMEM16A plays a role in the development of the mouse olfactory epithelium, we obtained the first immunohistochemistry study comparing the morphological properties of the olfactory epithelium and nasal glands in TMEM16A-/- and TMEM16A+/+ littermate mice. A comparison between the expression of the olfactory marker protein and adenylyl cyclase III shows that genetic ablation of TMEM16A did not seem to affect the maturation of olfactory sensory neurons and their ciliary layer. As TMEM16A is expressed at the apical part of supporting cells and in their microvilli, we used ezrin and cytokeratin 8 as markers of microvilli and cell body of supporting cells, respectively, and found that morphology and development of supporting cells were similar in TMEM16A-/- and TMEM16A+/+ littermate mice. The average number of supporting cells, olfactory sensory neurons, horizontal and globose basal cells were not significantly different in the two types of mice. Moreover, we also observed that the morphology of Bowman's glands, nasal septal glands and lateral nasal glands did not change in the absence of TMEM16A. Our results indicate that the development of mouse olfactory epithelium and nasal glands does not seem to be affected by the genetic ablation of TMEM16A.

Original languageEnglish
Article numbere0129171
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 11 2015

Fingerprint

Olfactory Mucosa
Ablation
Nose
Neurons
Olfactory Marker Protein
epithelium
Cells
Keratin-8
Chloride Channels
mice
Olfactory Receptor Neurons
sensory neurons
cells
Microvilli
microvilli
Physiological Phenomena
respiratory mucosa
chloride channels
Respiratory Mucosa
adenylate cyclase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Development of the olfactory epithelium and Nasal Glands in TMEM16A-/-and TMEM16A+/ +Mice. / Maurya, Devendra Kumar; Henriques, Tiago; Marini, Monica; Pedemonte, Nicoletta; Galietta, Luis J V; Rock, Jason R.; Harfe, Brian D.; Menini, Anna Menini.

In: PLoS One, Vol. 10, No. 6, e0129171, 11.06.2015.

Research output: Contribution to journalArticle

Maurya, Devendra Kumar ; Henriques, Tiago ; Marini, Monica ; Pedemonte, Nicoletta ; Galietta, Luis J V ; Rock, Jason R. ; Harfe, Brian D. ; Menini, Anna Menini. / Development of the olfactory epithelium and Nasal Glands in TMEM16A-/-and TMEM16A+/ +Mice. In: PLoS One. 2015 ; Vol. 10, No. 6.
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