TY - JOUR
T1 - Development of transplantable ascites tumours which continuously produce polyclonal antibodies in pristane primed BALB/c mice immunized with bacterial antigens and complete Freund's adjuvant
AU - Cevenini, Roberto
AU - Sambri, Vittorio
AU - Pileri, Stefano
AU - Ratti, Giulio
AU - La Placa, Michele
PY - 1991/6/24
Y1 - 1991/6/24
N2 - Bacterial immunogens (whole cells of Borrelia burgdorferi, elementary bodies of Chlamydia trachomatis and purified proteins of 22 and 24 kDa of Borrelia hermsii) were emulsified with an excess of complete Freund's adjuvant and injected (i.p.) on days 0, 7, 14 and 21, into BALB/c mice treated with pristane on day 6. This procedure induced the development of antibody-producing ascites tumours which could be serially transplanted in pristane-conditioned mice. Ascites tumours continued to yield a consistent amount of specific polyclonal antibody after ten serial transplants. The method described appears to be particularly useful for the production of a large amount of antibody when only small amounts of immunogen are available.
AB - Bacterial immunogens (whole cells of Borrelia burgdorferi, elementary bodies of Chlamydia trachomatis and purified proteins of 22 and 24 kDa of Borrelia hermsii) were emulsified with an excess of complete Freund's adjuvant and injected (i.p.) on days 0, 7, 14 and 21, into BALB/c mice treated with pristane on day 6. This procedure induced the development of antibody-producing ascites tumours which could be serially transplanted in pristane-conditioned mice. Ascites tumours continued to yield a consistent amount of specific polyclonal antibody after ten serial transplants. The method described appears to be particularly useful for the production of a large amount of antibody when only small amounts of immunogen are available.
KW - Ascites tumor
KW - BALB/c
KW - Borrelia burgdorferi
KW - Borrelia hermsii
KW - Chlamydia trachomatis
KW - Freund's adjuvant
KW - Polyclonal antibody production
KW - Pristane
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U2 - 10.1016/0022-1759(91)90132-Y
DO - 10.1016/0022-1759(91)90132-Y
M3 - Article
C2 - 2061607
AN - SCOPUS:0025877109
VL - 140
SP - 111
EP - 118
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -