Development of transplantable ascites tumours which continuously produce polyclonal antibodies in pristane primed BALB/c mice immunized with bacterial antigens and complete Freund's adjuvant

Roberto Cevenini, Vittorio Sambri, Stefano Pileri, Giulio Ratti, Michele La Placa

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial immunogens (whole cells of Borrelia burgdorferi, elementary bodies of Chlamydia trachomatis and purified proteins of 22 and 24 kDa of Borrelia hermsii) were emulsified with an excess of complete Freund's adjuvant and injected (i.p.) on days 0, 7, 14 and 21, into BALB/c mice treated with pristane on day 6. This procedure induced the development of antibody-producing ascites tumours which could be serially transplanted in pristane-conditioned mice. Ascites tumours continued to yield a consistent amount of specific polyclonal antibody after ten serial transplants. The method described appears to be particularly useful for the production of a large amount of antibody when only small amounts of immunogen are available.

Original languageEnglish
Pages (from-to)111-118
Number of pages8
JournalJournal of Immunological Methods
Volume140
Issue number1
DOIs
Publication statusPublished - Jun 24 1991

Keywords

  • Ascites tumor
  • BALB/c
  • Borrelia burgdorferi
  • Borrelia hermsii
  • Chlamydia trachomatis
  • Freund's adjuvant
  • Polyclonal antibody production
  • Pristane

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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