Developmental changes in glycopeptides synthesized by normal and dystrophic satellite cells in culture

G. Cossu, M. Pacifici, M. Marino, B. Zani, M. Coletta, M. Molinaro

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Abstract

Satellite cells were isolated from adult posterior leg muscles of normal and dystrophic (C57 BL/6J/dydy) mice and were grown in culture conditions which allow their terminal differentiation into multinucleated myotubes. Biosynthesis of total cell glycoproteins was studied in normal and dystrophic satellite cells at different stages of cytodifferentiation in vitro by labelling with radioactive fucose and glucosamine. Total radiolabelled glycoproteins were digested with pronase and the resulting glycopeptides were analyzed by gel filtration on Sephadex G-50 and by ion exchange chromatography on DEAE-cellulose. With these techniques, total glycopeptides could be separated into several classes according to their average molecular size and anionic charge. The results obtained show that qualitative and quantitative changes in several classes of glycopeptides occur during cytodifferentiation in vitro of satellite cells from both normal and dystrophic mice. In terms of qualitative changes, a class of fucosyl-glycopeptides, which is eluted at 22 mM sodium phosphate from DEAE-cellulose columns, appears to be exclusively synthesized by normal multinucleated myotubes but not by duplicating mononucleated satellite cells or by other non-myogenic cells. On the other hand, a similar but not identical class of fucosyl-glycopeptides, eluted at 20 mM sodium phosphate from DEAE-cellulose columns, was found to be exclusively synthesized by multinucleated myotubes derived from dystrophic mice. No other differences were found when comparing myotubes from normal vs dystrophic mice.

Original languageEnglish
Pages (from-to)349-357
Number of pages9
JournalExperimental Cell Research
Volume132
Issue number2
DOIs
Publication statusPublished - 1981

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Glycopeptides
Cell Culture Techniques
Skeletal Muscle Fibers
DEAE-Cellulose
Glycoproteins
Pronase
Fucose
Glucosamine
Ion Exchange Chromatography
Gel Chromatography
Leg
Muscles

ASJC Scopus subject areas

  • Cell Biology

Cite this

Developmental changes in glycopeptides synthesized by normal and dystrophic satellite cells in culture. / Cossu, G.; Pacifici, M.; Marino, M.; Zani, B.; Coletta, M.; Molinaro, M.

In: Experimental Cell Research, Vol. 132, No. 2, 1981, p. 349-357.

Research output: Contribution to journalArticle

Cossu, G. ; Pacifici, M. ; Marino, M. ; Zani, B. ; Coletta, M. ; Molinaro, M. / Developmental changes in glycopeptides synthesized by normal and dystrophic satellite cells in culture. In: Experimental Cell Research. 1981 ; Vol. 132, No. 2. pp. 349-357.
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