Developmental expression of PC3 gene is correlated with neuronal cell birthday

Paola Iacopetti, Giuseppina Barsacchi, Felice Tirone, Lamberto Maffei, Federico Cremisi

Research output: Contribution to journalArticle

Abstract

We examined the developmental expression of PC3, a nerve growth factor (NGF) early induced gene in PC12 cells, in the rat central nervous system (CNS) and we found that it represents a molecular marker of ongoing postmitotic neurons production. PC3 is initially expressed in the ventral quarter of the neural tube, at the level of the presumptive cervical spinal cord just where and when (10-11 days post coitum (dpc)) the motor neurons are arising. Subsequently, the appearance of PC3 expression follows a ventro-dorsal and a rosto-caudal gradient in the spinal cord and a caudo-rostal gradient across the brain vesicles that coincide, both spatially and temporally, with the gradients of neurogenesis described in the literature. As in PC12 cells, PC3 mRNA expression appears to be transient in vivo. In all regions of the CNS, it is restricted to the ventricular zone of the neuroepithelium, while neuronal precursors cease to express PC3 as they migrate to the mantle zone. Moreover, PC3 mRNA disappears from the various regions of the CNS as neurogenesis ceases.

Original languageEnglish
Pages (from-to)127-137
Number of pages11
JournalMechanisms of Development
Volume47
Issue number2
DOIs
Publication statusPublished - 1994

Keywords

  • In situ hybridization
  • Neurogenesis
  • Neuronal cell birthday
  • PC3

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

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