Developmental expression of somatostatin in mouse brain. II. In situ hybridization

Caterina Bendotti, Christine Hohmann, Gianluigi Forloni, Roger Reeves, Joseph T. Coyle, Mary Lou Oster-Granite

Research output: Contribution to journalArticle

Abstract

The distribution and the levels of expression of preprosomatostatin (PPSOM) mRNA were examined during pre- and postnatal development of the mouse brain using the in situ hybridization technique. The signal obtained by in situ hybridization of embryonic tissues at day 14 and day 17 of gestation was highest over the neurons of the pyriform cortex, amygdala, and entopeduncular nucleus. The signal was very low over cells of the neocortex and the developing hippocampal formation. The density of grains overlying the neurons of the amygdala and pyriform cortex continued to be high during early postnatal life, but decreased as the animals became adults. A progressive increase of PPSOM mRNA expression was observed in postnatal animals in the stratum oriens and dentate gyrus of the hippocampal formation. In the cerebral cortex and striatum, the number of these neurons became maximal between postnatal weeks 1 and 3. In the diencephalon, the highest densities of grains were found over neurons in the nucleus reticularis thalami and zona incerta at postnatal day 21; these levels declined slightly thereafter. The cells of the periventricular nucleus of the hypothalamus had high densities of grains as early as postnatal week 1 and continued to have high densities of grains in adult animals. These patterns of hybridization density parallelled the distribution of SOM-like immunoreactivity in the mouse brain, When PPSOM mRNA expression was examined in the cerebral cortices of mice that received lesions of the nucleus basalis of Meynert as neonates, a transient increase in the number of cells expressing PPSOM mRNA was observed in the frontoparietal cortex ipsilateral to the lesion at postnatal day 10, but not at postnatal day 30. Importantly, the density of grains over the individual cells was not altered in lesioned animals at these two ages.

Original languageEnglish
Pages (from-to)26-39
Number of pages14
JournalDevelopmental Brain Research
Volume53
Issue number1
DOIs
Publication statusPublished - Apr 1 1990

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Somatostatin
In Situ Hybridization
Neurons
Messenger RNA
Brain
Amygdala
Cerebral Cortex
Hippocampus
Entopeduncular Nucleus
Basal Nucleus of Meynert
Diencephalon
Neocortex
Dentate Gyrus
Cell Nucleus
Thalamus
Hypothalamus
Cell Count
Pregnancy
Piriform Cortex

Keywords

  • Development
  • Preprosomatostatin
  • Somatostatin

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Developmental expression of somatostatin in mouse brain. II. In situ hybridization. / Bendotti, Caterina; Hohmann, Christine; Forloni, Gianluigi; Reeves, Roger; Coyle, Joseph T.; Oster-Granite, Mary Lou.

In: Developmental Brain Research, Vol. 53, No. 1, 01.04.1990, p. 26-39.

Research output: Contribution to journalArticle

Bendotti, Caterina ; Hohmann, Christine ; Forloni, Gianluigi ; Reeves, Roger ; Coyle, Joseph T. ; Oster-Granite, Mary Lou. / Developmental expression of somatostatin in mouse brain. II. In situ hybridization. In: Developmental Brain Research. 1990 ; Vol. 53, No. 1. pp. 26-39.
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abstract = "The distribution and the levels of expression of preprosomatostatin (PPSOM) mRNA were examined during pre- and postnatal development of the mouse brain using the in situ hybridization technique. The signal obtained by in situ hybridization of embryonic tissues at day 14 and day 17 of gestation was highest over the neurons of the pyriform cortex, amygdala, and entopeduncular nucleus. The signal was very low over cells of the neocortex and the developing hippocampal formation. The density of grains overlying the neurons of the amygdala and pyriform cortex continued to be high during early postnatal life, but decreased as the animals became adults. A progressive increase of PPSOM mRNA expression was observed in postnatal animals in the stratum oriens and dentate gyrus of the hippocampal formation. In the cerebral cortex and striatum, the number of these neurons became maximal between postnatal weeks 1 and 3. In the diencephalon, the highest densities of grains were found over neurons in the nucleus reticularis thalami and zona incerta at postnatal day 21; these levels declined slightly thereafter. The cells of the periventricular nucleus of the hypothalamus had high densities of grains as early as postnatal week 1 and continued to have high densities of grains in adult animals. These patterns of hybridization density parallelled the distribution of SOM-like immunoreactivity in the mouse brain, When PPSOM mRNA expression was examined in the cerebral cortices of mice that received lesions of the nucleus basalis of Meynert as neonates, a transient increase in the number of cells expressing PPSOM mRNA was observed in the frontoparietal cortex ipsilateral to the lesion at postnatal day 10, but not at postnatal day 30. Importantly, the density of grains over the individual cells was not altered in lesioned animals at these two ages.",
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