Developmental expression of the gene encoding growth-associated protein 43 (GAP43) in the brains of normal and aneuploid mice

G. T. Capone, C. Bendotti, M. L. Oster-Granite, J. T. Coyle

Research output: Contribution to journalArticle

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Abstract

The gene encoding growth-associated protein 43 (Gap43), a neuronal phosphoprotein associated with axonal outgrowth and synaptic plasticity, is located on mouse chromosome 16 (MMU16). We examined the developmental expression of Gap43 in normal, trisomy 16 (Ts16), and trisomy 19 (Ts19) mouse brain using northern blot analysis and in situ hybridization as a first step toward understanding the neurobiologic consequences of increased gene dosage on brain development. Gap43 expression was detected by in situ hybridization throughout the mesencephalon, rhombencephalon, spinal cord, and first branchial arch in whole embryos as early as day 10 of gestation (E10). By E15, Gap43 expression was localized to cells in the retina, the olfactory bulbs, and anterior olfactory structures, the cortical plate, the basal telencephalon, diencephalon, midbrain, hindbrain, and spinal cord. Northern blot analysis detected a three-fold increase in Gap43 mRNA levels in the brains of normal mice between E12-E18. At E15, Gap43 mRNA levels were increased 35-40% in Ts16 mouse brain and decreased 10% in Ts19 mouse brain, relative to euploid littermate controls. Using in situ hybridization we found that overexpression of Gap43 occurred in the diencephalon, medial and lateral basal telencephalon, and cortical plate region in Ts16 mice relative to littermate controls. Thus, the degree of overexpression of Gap43 mRNA in Ts16 mice is consistent with that expected from gene dosage effects.

Original languageEnglish
Pages (from-to)449-460
Number of pages12
JournalJournal of Neuroscience Research
Volume29
Issue number4
DOIs
Publication statusPublished - 1991

Fingerprint

GAP-43 Protein
Developmental Genes
Aneuploidy
Brain
In Situ Hybridization
Diencephalon
Telencephalon
Rhombencephalon
Gene Dosage
Trisomy
Mesencephalon
Northern Blotting
Cerebral Cortex
Messenger RNA
Spinal Cord
Branchial Region
Chromosomes, Human, Pair 16
Neuronal Plasticity
Olfactory Bulb
Phosphoproteins

Keywords

  • aneuploidy
  • axonal outgrowth
  • Gap43
  • gene expression
  • trisomy 16 mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Developmental expression of the gene encoding growth-associated protein 43 (GAP43) in the brains of normal and aneuploid mice. / Capone, G. T.; Bendotti, C.; Oster-Granite, M. L.; Coyle, J. T.

In: Journal of Neuroscience Research, Vol. 29, No. 4, 1991, p. 449-460.

Research output: Contribution to journalArticle

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