Developmental regulation of Lck targeting to the CD8 coreceptor controls signaling in naive and memory T cells

Martin F. Bachmann, Awen Gallimore, Susanne Linkert, Vincenzo Cerundolo, Antonio Lanzavecchia, Manfred Kopf, Antonella Viola

Research output: Contribution to journalArticle

Abstract

The question of whether enhanced memory T cell responses are simply due to an increased frequency of specific cells or also to an improved response at the single cell level is widely debated. In this study, we analyzed T cell receptor (TCR) transgenic memory T cells and bona fide memory T cells isolated from virally infected normal mice using the tetramer technology. We found that memory T cells are qualitatively different from naive T cells due to a developmentally regulated rearrangement of the topology of the signaling machinery. In naive cytotoxic T cells, only a few CD8 molecules are associated with Lck and the kinase is homogeneously distributed inside the cell. However, in vivo priming of naive T cells induces the targeting of Lck to the CD8 coreceptor in the cell membrane and the consequent organization of a more efficient TCR signaling machinery in effector and memory cells.

Original languageEnglish
Pages (from-to)1521-1529
Number of pages9
JournalJournal of Experimental Medicine
Volume189
Issue number10
DOIs
Publication statusPublished - May 17 1999

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T-Lymphocytes
T-Cell Antigen Receptor
Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
Cell Membrane
Technology

Keywords

  • Costimulation
  • Memory
  • Virus

ASJC Scopus subject areas

  • Immunology

Cite this

Bachmann, M. F., Gallimore, A., Linkert, S., Cerundolo, V., Lanzavecchia, A., Kopf, M., & Viola, A. (1999). Developmental regulation of Lck targeting to the CD8 coreceptor controls signaling in naive and memory T cells. Journal of Experimental Medicine, 189(10), 1521-1529. https://doi.org/10.1084/jem.189.10.1521

Developmental regulation of Lck targeting to the CD8 coreceptor controls signaling in naive and memory T cells. / Bachmann, Martin F.; Gallimore, Awen; Linkert, Susanne; Cerundolo, Vincenzo; Lanzavecchia, Antonio; Kopf, Manfred; Viola, Antonella.

In: Journal of Experimental Medicine, Vol. 189, No. 10, 17.05.1999, p. 1521-1529.

Research output: Contribution to journalArticle

Bachmann, MF, Gallimore, A, Linkert, S, Cerundolo, V, Lanzavecchia, A, Kopf, M & Viola, A 1999, 'Developmental regulation of Lck targeting to the CD8 coreceptor controls signaling in naive and memory T cells', Journal of Experimental Medicine, vol. 189, no. 10, pp. 1521-1529. https://doi.org/10.1084/jem.189.10.1521
Bachmann, Martin F. ; Gallimore, Awen ; Linkert, Susanne ; Cerundolo, Vincenzo ; Lanzavecchia, Antonio ; Kopf, Manfred ; Viola, Antonella. / Developmental regulation of Lck targeting to the CD8 coreceptor controls signaling in naive and memory T cells. In: Journal of Experimental Medicine. 1999 ; Vol. 189, No. 10. pp. 1521-1529.
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