Diagnosis of paternity in granulocytes from peripheral blood: Fluorescence study using quinacrine (Italian)

Drumstick like appendages and large male sex chromatin bodies were found in quinacrine stained polymorphonuclear leucocytes of a patient carrying a long Y chromosome, the size of an E group chromosome. The male sex chromatin bodies in the alleged father were smaller than in the patient and his Y chromosome had the size of a G group chromosome. The paternity could be excluded on the basis of these observations, which subsequently were confirmed by the blood group determinations and by the mother's acknowledgement.