Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens

Laura Selan, Claudio Passariello, Luigi Rizzo, Paola Varesi, Francesco Speziale, Giulio Renzini, Maria Cristina Thaller, Paolo Fiorani, Gian Maria Rossolini

Research output: Contribution to journalArticle

Abstract

Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients in other groups. Antibody titres of 0.40 ELISA units (EU) or more, or 0.35 EU or more detected 97% and 100% of staphylococcal LO-SVGIs, respectively, 0% and 2% titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.

Original languageEnglish
Pages (from-to)2166-2168
Number of pages3
JournalLancet
Volume359
Issue number9324
DOIs
Publication statusPublished - Jun 22 2002

Fingerprint

Blood Vessels
Enzyme-Linked Immunosorbent Assay
Polysaccharides
Transplants
Antigens
Antibodies
Infection
Biofilms
Staphylococcus
Routine Diagnostic Tests
Immunoglobulin M
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Selan, L., Passariello, C., Rizzo, L., Varesi, P., Speziale, F., Renzini, G., ... Rossolini, G. M. (2002). Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens. Lancet, 359(9324), 2166-2168. https://doi.org/10.1016/S0140-6736(02)09086-4

Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens. / Selan, Laura; Passariello, Claudio; Rizzo, Luigi; Varesi, Paola; Speziale, Francesco; Renzini, Giulio; Thaller, Maria Cristina; Fiorani, Paolo; Rossolini, Gian Maria.

In: Lancet, Vol. 359, No. 9324, 22.06.2002, p. 2166-2168.

Research output: Contribution to journalArticle

Selan, L, Passariello, C, Rizzo, L, Varesi, P, Speziale, F, Renzini, G, Thaller, MC, Fiorani, P & Rossolini, GM 2002, 'Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens', Lancet, vol. 359, no. 9324, pp. 2166-2168. https://doi.org/10.1016/S0140-6736(02)09086-4
Selan L, Passariello C, Rizzo L, Varesi P, Speziale F, Renzini G et al. Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens. Lancet. 2002 Jun 22;359(9324):2166-2168. https://doi.org/10.1016/S0140-6736(02)09086-4
Selan, Laura ; Passariello, Claudio ; Rizzo, Luigi ; Varesi, Paola ; Speziale, Francesco ; Renzini, Giulio ; Thaller, Maria Cristina ; Fiorani, Paolo ; Rossolini, Gian Maria. / Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens. In: Lancet. 2002 ; Vol. 359, No. 9324. pp. 2166-2168.
@article{5668110bf9bb4ab4894b39065a42d176,
title = "Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens",
abstract = "Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients in other groups. Antibody titres of 0.40 ELISA units (EU) or more, or 0.35 EU or more detected 97{\%} and 100{\%} of staphylococcal LO-SVGIs, respectively, 0{\%} and 2{\%} titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.",
author = "Laura Selan and Claudio Passariello and Luigi Rizzo and Paola Varesi and Francesco Speziale and Giulio Renzini and Thaller, {Maria Cristina} and Paolo Fiorani and Rossolini, {Gian Maria}",
year = "2002",
month = "6",
day = "22",
doi = "10.1016/S0140-6736(02)09086-4",
language = "English",
volume = "359",
pages = "2166--2168",
journal = "The Lancet",
issn = "0140-6736",
publisher = "Lancet Publishing Group",
number = "9324",

}

TY - JOUR

T1 - Diagnosis of vascular graft infections with antibodies against staphylococcal slime antigens

AU - Selan, Laura

AU - Passariello, Claudio

AU - Rizzo, Luigi

AU - Varesi, Paola

AU - Speziale, Francesco

AU - Renzini, Giulio

AU - Thaller, Maria Cristina

AU - Fiorani, Paolo

AU - Rossolini, Gian Maria

PY - 2002/6/22

Y1 - 2002/6/22

N2 - Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients in other groups. Antibody titres of 0.40 ELISA units (EU) or more, or 0.35 EU or more detected 97% and 100% of staphylococcal LO-SVGIs, respectively, 0% and 2% titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.

AB - Late-onset infections of synthetic vascular grafts (LO-SVGIs) are generally caused by staphylococci that produce a slime polysaccharide and grow as a biofilm on the graft surface. We developed an ELISA to detect serum antibodies against staphylococcal slime polysaccharide antigens (SSPA). Patients with an ongoing staphylococcal LO-SVGI had greater titres of IgM antibodies against SSPA than did patients in other groups. Antibody titres of 0.40 ELISA units (EU) or more, or 0.35 EU or more detected 97% and 100% of staphylococcal LO-SVGIs, respectively, 0% and 2% titre/unit false-positive results. Our findings suggest that such an ELISA represents a sensitive, specific, and non-invasive diagnostic test for staphylococcal LO-SVGIs.

UR - http://www.scopus.com/inward/record.url?scp=0037157584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037157584&partnerID=8YFLogxK

U2 - 10.1016/S0140-6736(02)09086-4

DO - 10.1016/S0140-6736(02)09086-4

M3 - Article

C2 - 12090985

AN - SCOPUS:0037157584

VL - 359

SP - 2166

EP - 2168

JO - The Lancet

JF - The Lancet

SN - 0140-6736

IS - 9324

ER -