Diagnostic and prognostic value of molecular and serological investigation of human parvovirus B19 infection during pregnancy

Maurizio Zavattoni, Stefano Paolucci, Antonella Sarasini, Beatrice Tassis, Mariangela Rustico, Aida Quarenghi, Antonio Piralla, Fausto Baldanti

Research output: Contribution to journalArticle

Abstract

To define diagnostic and prognostic markers of parvovirus B19 (B19V) fetal infection, two groups were investigated: 1) pregnant women with specific symptoms or contacts with symptomatic households (n=37); 2) mothers with pathological ultrasound findings and the relevant fetus at the time of prenatal diagnosis (n=16). In the first group, diagnosis of B19V infection was achieved using IgM detection in 29/37 (78.3%) of patients, while B19V DNA was detected in 36/37 (97.3%) of infected women. In the second group, intrauterine infection was investigated by amniocentesis (n=5), cordocentesis (n=3) or both (n=5). Median B19V DNA load in amniotic fluid was 8.2×107 copies/ml and in fetal blood was 2×109 copies/ml. Maternal blood was positive for B19V DNA (median 3.8×104 copies/ml) in 14/16 (87.5%) women examined. At time of fetal US investigation, all mothers were B19V IgG positive and B19V IgM were detected in 10/16 (62.5%), while fetal B19V IgG and IgM were detected in 1/8 (12.5%) and 5/8 (62.5%), respectively. Phylogenetic analysis revealed that all B19V maternal and fetal strains belonged to genotype 1A. Diagnosis of maternal, fetal and neonatal B19V infection should be based on both IgM and DNA detection. Prognostic markers of congenital B19V infection need to be defined.

Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalNew Microbiologica
Volume39
Issue number3
Publication statusPublished - Apr 1 2016

Keywords

  • Fetal and neonatal diagnosis
  • Maternal
  • Parvovirus B19

ASJC Scopus subject areas

  • Medicine(all)
  • Microbiology (medical)

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