TY - JOUR
T1 - Diagnostic identification of malignant cells in the cerebrospinal fluid by tumor-specific qRT-PCR
AU - Rosanda, Cristina
AU - Gambini, Claudio
AU - Carlini, Barbara
AU - Conte, Massimo
AU - De Bernardi, Bruno
AU - Garaventa, Alberto
AU - Corrias, Maria Valeria
PY - 2006/6
Y1 - 2006/6
N2 - Tumor specific quantitative RT-PCRs for two neuroblastoma specific molecular markers, tyrosine hydroxylase (TH) and GD2 synthase, were used to unequivocally demonstrate the neoplastic nature of the cells present in the cerebrospinal fluid of a neuroblastoma patient. After radical surgery of two separate tumoral lesions, localized in the extradural area, the patient presented with meningitis. Common sites of neuroblastoma metastatization, e.g. bone and bone marrow, were not infiltrated by tumor cells, as assessed by standard scintigraphy, morphological investigation and by sensitive and specific immunocytochemical and molecular assays. The results presented here demonstrate the successful use of tumor-specific qRT-PCRs in cerebrospinal fluid to investigate questionable clinical cases. The technique, which compared to other detection methods (e.g., immunocytochemistry) requires very few cells, yields unambiguous information once a suspected diagnosis has been formulated and a tumor-specific molecular marker is available.
AB - Tumor specific quantitative RT-PCRs for two neuroblastoma specific molecular markers, tyrosine hydroxylase (TH) and GD2 synthase, were used to unequivocally demonstrate the neoplastic nature of the cells present in the cerebrospinal fluid of a neuroblastoma patient. After radical surgery of two separate tumoral lesions, localized in the extradural area, the patient presented with meningitis. Common sites of neuroblastoma metastatization, e.g. bone and bone marrow, were not infiltrated by tumor cells, as assessed by standard scintigraphy, morphological investigation and by sensitive and specific immunocytochemical and molecular assays. The results presented here demonstrate the successful use of tumor-specific qRT-PCRs in cerebrospinal fluid to investigate questionable clinical cases. The technique, which compared to other detection methods (e.g., immunocytochemistry) requires very few cells, yields unambiguous information once a suspected diagnosis has been formulated and a tumor-specific molecular marker is available.
KW - Cancer
KW - CSF
KW - Detection
KW - GD2 synthase
KW - Neuroblastoma
KW - Tyrosine hydroxylase
UR - http://www.scopus.com/inward/record.url?scp=33750564272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33750564272&partnerID=8YFLogxK
U2 - 10.1007/s10585-006-9032-z
DO - 10.1007/s10585-006-9032-z
M3 - Article
C2 - 17028920
AN - SCOPUS:33750564272
VL - 23
SP - 223
EP - 226
JO - Clinical and Experimental Metastasis
JF - Clinical and Experimental Metastasis
SN - 0262-0898
IS - 3-4
ER -