Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories

Marcus Panning, Thomas Laue, Stephan Ölschlager, Markus Eickmann, Stephan Becker, Sabine Raith, Marie Claude Georges Courbot, Mikael Nilsson, Robin Gopal, Ake Lundkvist, Antonino Di Caro, David Brown, Hermann Meyer, Graham Lloyd, Beate M. Kümmerer, Stephan Günther, Christian Drosten

Research output: Contribution to journalArticle

Abstract

A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL; Cote d'Ivoire ebolavirus, 537 copies/mL; Reston ebolavirus, 4546 copies/mL; Lake Victoria marburgvirus Musoke, 860 copies/mL; and Lake Victoria marburgvirus Ravn, 1551 copies/mL. The assay facilitates reliable detection or exclusion screening of filovirus infections.

Original languageEnglish
JournalJournal of Infectious Diseases
Volume196
Issue numberSUPPL. 2
DOIs
Publication statusPublished - Nov 15 2007

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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    Panning, M., Laue, T., Ölschlager, S., Eickmann, M., Becker, S., Raith, S., Courbot, M. C. G., Nilsson, M., Gopal, R., Lundkvist, A., Di Caro, A., Brown, D., Meyer, H., Lloyd, G., Kümmerer, B. M., Günther, S., & Drosten, C. (2007). Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories. Journal of Infectious Diseases, 196(SUPPL. 2). https://doi.org/10.1086/520600