Diagnostic value of PRND gene expression profiles in astrocytomas: Relationship to tumor grades of malignancy

Sergio Comincini, V. Ferrara, A. Arias, A. Malovini, A. Azzalin, L. Ferretti, E. Benericetti, M. Cardarelli, M. Gerosa, M. G. Passarin, S. Turazzi, R. Bellazzi

Research output: Contribution to journalArticlepeer-review

Abstract

Doppel (PRND) is a paralogue of the mammalian prion (PRNP) gene. It is abundant in testis and, unlike PRNP, it is expressed at low levels in the adult central nervous system (CNS). Besides, doppel overexpression correlates with some prion-disease pathological features, such as ataxia and death of cerebellar neurons. Recently, ectopic expression of doppel was found in two different tumor types, specifically in glial and haematological cancers. In order to address clinical important issues, PRND mRNA expression was investigated in a panel of 111 astrocytoma tissue samples, histologically classified according to the World Health Organization (WHO) criteria (6 grade I pilocytic astrocytomas, 15 grade II low-grade astrocytomas, 26 grade III anaplastic astrocytomas and 64 grade IV glioblastoma multiforme). Real-time PRND gene expression profiling, after normalisation with GAPDH, revealed large differences between low (WHO I and II) and high grade (III and IV) of malignancy (P

Original languageEnglish
Pages (from-to)989-996
Number of pages8
JournalOncology Reports
Volume17
Issue number5
Publication statusPublished - May 2007

Keywords

  • Astrocytoma
  • Gene expression
  • Molecular diagnosis
  • Real-time PCR analysis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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