Dictyostelium cells produce platelet-activating factor in response to cAMP

F. Bussolino, C. Sordano, E. Benfenati, S. Bozzaro

Research output: Contribution to journalArticlepeer-review

Abstract

Evidence is provided that Dictyostelium discoideum cells produce 1-O-alkyl-2-δ-acetyl-O-sn-glycero-3-phosphocholine (platelet-activating factor, PAF). D. discoideum PAF has been characterized as being identical with mammalian platelet-activating factor, based on its stimulation of rabbit platelet aggregation, its physico-chemical properties and mass spectrum. The basal activity of PAF increases after starvation and during aggregation and declines at the slug stage. PAF is not detected in the extracellular space. Cell treatment with cAMP pulses stimulates a transient accumulation of PAF, probably via activation of a cAMP-dependent acetyltransferase, suggesting a possible involvement of PAF in cAMP-regulated processes in Dictyostelium.

Original languageEnglish
Pages (from-to)609-615
Number of pages7
JournalEuropean Journal of Biochemistry
Volume196
Issue number3
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Biochemistry

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