Diepoxybutane and mitomycin C toxicity is associated with the induction of oxidative DNA damage in sea urchin embryos

G. Pagano, P. Degan, A. De Biase, M. Iaccarino, M. Warnau

Research output: Contribution to journalArticlepeer-review

Abstract

Diepoxybutane (DEB)- and mitomycin C (MMC)-associated toxicity was investigated in embryos from the sea urchin (SU) species Sphaerechinus granularis. DEB- and MMC-induced toxicity resulted in S. granularis embryos and larvae at concentrations ranging 10-5 to 10-4 M DEB, and 3×10-6 to 3×10-5 M MMC, in terms of larval malformations, developmental arrest and mortality. The formation of DNA oxidative damage, 8-hydroxy-2′-deoxyguanosine (8-OHdG) was measured in DEB- and in MMC-exposed embryos (at gastrula stage). A dose-dependent increase in 8-OHdG levels was observed that was significantly correlated with DEB- and MMC-induced developmental defects. The results lend further support to the body of evidence associating both DEB and MMC toxicity with oxidative stress, including DNA oxidative damage.

Original languageEnglish
Pages (from-to)651-655
Number of pages5
JournalHuman and Experimental Toxicology
Volume20
Issue number12
DOIs
Publication statusPublished - 2001

Keywords

  • 8-hydroxy-2′-deoxyguanosine
  • Developmental defects
  • Diepoxybutane
  • DNA oxidative damage
  • Fanconi's anaemia
  • Mitomycin C
  • Sea urchins

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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