TY - JOUR
T1 - Dietary magnesium alleviates experimental murine colitis through modulation of gut microbiota
AU - Del Chierico, Federica
AU - Trapani, Valentina
AU - Petito, Valentina
AU - Reddel, Sofia
AU - Pietropaolo, Giuseppe
AU - Graziani, Cristina
AU - Masi, Letizia
AU - Gasbarrini, Antonio
AU - Putignani, Lorenza
AU - Scaldaferri, Franco
AU - Wolf, Federica I.
N1 - Funding Information:
Funding: F.I.W. acknowledges funding by MIUR (Italian Ministry of University and Research) D.3.2-2015 and D.1.2017.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - Nutritional deficiencies are common in inflammatory bowel diseases (IBD). In patients, magnesium (Mg) deficiency is associated with disease severity, while in murine models, dietary Mg supplementation contributes to restoring mucosal function. Since Mg availability modulates key bacterial functions, including growth and virulence, we investigated whether the beneficial effects of Mg supplementation during colitis might be mediated by gut microbiota. The effects of dietary Mg modulation were assessed in a murine model of dextran sodium sulfate (DSS)-induced colitis by monitoring magnesemia, weight, and fecal consistency. Gut microbiota were analyzed by 16S-rRNA based profiling on fecal samples. Mg supplementation improved microbiota richness in colitic mice, increased abundance of Bifidobacterium and reduced Enterobacteriaceae. KEEG pathway analysis predicted an increase in biosynthetic metabolism, DNA repair and translation pathways during Mg supplementation and in the presence of colitis, while low Mg conditions favored catabolic processes. Thus, dietary Mg supplementation increases bacteria involved in intestinal health and metabolic homeostasis, and reduces bacteria involved in inflammation and associated with human diseases, such as IBD. These findings suggest that Mg supplementation may be a safe and cost-effective strategy to ameliorate disease symptoms and restore a beneficial intestinal flora in IBD patients.
AB - Nutritional deficiencies are common in inflammatory bowel diseases (IBD). In patients, magnesium (Mg) deficiency is associated with disease severity, while in murine models, dietary Mg supplementation contributes to restoring mucosal function. Since Mg availability modulates key bacterial functions, including growth and virulence, we investigated whether the beneficial effects of Mg supplementation during colitis might be mediated by gut microbiota. The effects of dietary Mg modulation were assessed in a murine model of dextran sodium sulfate (DSS)-induced colitis by monitoring magnesemia, weight, and fecal consistency. Gut microbiota were analyzed by 16S-rRNA based profiling on fecal samples. Mg supplementation improved microbiota richness in colitic mice, increased abundance of Bifidobacterium and reduced Enterobacteriaceae. KEEG pathway analysis predicted an increase in biosynthetic metabolism, DNA repair and translation pathways during Mg supplementation and in the presence of colitis, while low Mg conditions favored catabolic processes. Thus, dietary Mg supplementation increases bacteria involved in intestinal health and metabolic homeostasis, and reduces bacteria involved in inflammation and associated with human diseases, such as IBD. These findings suggest that Mg supplementation may be a safe and cost-effective strategy to ameliorate disease symptoms and restore a beneficial intestinal flora in IBD patients.
KW - Bifidobacterium
KW - Dextran sodium sulfate
KW - Dysbiosis
KW - Enterobacteriacee
KW - Inflammatory bowel disease
KW - Magnesemia
KW - Magnesium supplementation
KW - Metabolism
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U2 - 10.3390/nu13124188
DO - 10.3390/nu13124188
M3 - Article
AN - SCOPUS:85119614264
VL - 13
JO - Nutrients
JF - Nutrients
SN - 2072-6643
IS - 12
M1 - 4188
ER -