Differences between semen and plasma of nucleoside reverse transcriptase resistance mutations in HIV-infected patients, using a rapid assay

Giuseppina Liuzzi, Antonio Chirianni, Mauro Zaccarelli, Daniela Zinzi, Vincenzo Esposito, Vincenzo Guadagnino, Andrea Antinori, Marcello Piazza

Research output: Contribution to journalArticlepeer-review

Abstract

The emergence of HIV-resistant strains generally occurs during treatment failure. A high rate of viral replication, low patient adherence to therapy, poor drug absorption or increased drug metabolism may be related to treatment failure and favour the onset of resistance. Recently, despite the absence of detectable HIV viral load in plasma, the importance of the persistence of HIV replication in other reservoirs has been underlined. Paired semen and plasma specimens stored from HIV male patients were tested using the LiPA assay, detecting genotype mutations related to Nucleoside Reverse Transciptase Inhibitors (NRTI) resistance. Overall, 32 paired semen and plasma samples were analyzed. The presence of HTV sequences was determined in 13 paired specimens (40.6%). Sixteen semen and 10 plasma specimens failed to amplify. At least one mutation was identified in the plasma of 77.8% of treated and 50.0% of naïve patients. Similarly, mutations were identified in the semen of 66.7% of treated and 50.0% of untreated patients. Different mutation patterns between the two compartments were found in 46.2% of patients. The use of rapid assessment of HIV resistance in plasma and semen may be useful in some situations, such as HIV infection transmitted by sexual contact. Our data reinforce the evidence that, regarding HIV replication, the male genital tract must be considered as a separate compartment from the plasma.

Original languageEnglish
Pages (from-to)509-512
Number of pages4
JournalIn Vivo
Volume18
Issue number4
Publication statusPublished - Jul 2004

Keywords

  • Drug resistance
  • HIV-1
  • LIPA
  • Semen

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Differences between semen and plasma of nucleoside reverse transcriptase resistance mutations in HIV-infected patients, using a rapid assay'. Together they form a unique fingerprint.

Cite this