Differences in corneocyte surface area in pre- and post-menopausal women: Assessment with the noninvasive Videomicroscopic Imaging of Corneocytes Method (VIC) under basal conditions

Joachim W. Fluhr, A. Pelosi, S. Lazzerini, S. Dikstein, E. Berardesca

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The differences between pre- and postmenopausal women and men on corneocyte surface area were investigated by a noninvasive exfoliation method. Surface corneocytes were collected with a modified detergent scrub technique. Separated corneocytes were analyzed by videomicroscopy and image analyses (NIH Image™ 1.59). Additionally transepidermal water loss (TEWL) and stratum corneum hydration, water-holding capacity and moisture accumulation velocity were measured. 21 postmenopausal females without hormonal substitution (age 50.6 years), 33 premenopausal women (age 41.0 years) and 25 men matched for age (age 44.0 years) were included in this study. The smallest corneocyte surface area was seen in premenopausal women (368.0 pixels). For postmenopausal women and the male control population almost equal values could be detected (postmenopausal women 401.1 pixels; men 401.8 pixels). Significant differences were calculated with the ANOVA test (p = 0.0050) and post-hoc analyses (Dunn test); premenopausal vs. postmenopausal women (p <0.05) and premenopausal women vs. men (p <0.05), but not postmenopausal women vs. men (p > 0.05). No statistically significant differences could be detected for TEWL, stratum corneum hydration parameters. No correlation could be found between the corneocyte surface area and barrier or hydration parameters. The detected differences support the hypothesis that sexual hormones have an impact on corneocyte surface area, because sex hormone levels are higher in premenopausal women than in non-hormone-substituted postmenopausal women or men.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalSkin Pharmacology and Applied Skin Physiology
Volume14
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2001

Fingerprint

Cornea
Water
Video Microscopy
Gonadal Steroid Hormones
Detergents
Analysis of Variance
Hormones
Population

Keywords

  • Barrier function
  • Corneocyte surface area
  • Hydration parameters
  • Menopause
  • Sex hormones
  • Stratum corneum
  • Videomicroscopy

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Dermatology
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Differences in corneocyte surface area in pre- and post-menopausal women : Assessment with the noninvasive Videomicroscopic Imaging of Corneocytes Method (VIC) under basal conditions. / Fluhr, Joachim W.; Pelosi, A.; Lazzerini, S.; Dikstein, S.; Berardesca, E.

In: Skin Pharmacology and Applied Skin Physiology, Vol. 14, No. SUPPL. 1, 2001, p. 10-16.

Research output: Contribution to journalArticle

@article{eb619113f39e400fa7b7d0cad46d19a0,
title = "Differences in corneocyte surface area in pre- and post-menopausal women: Assessment with the noninvasive Videomicroscopic Imaging of Corneocytes Method (VIC) under basal conditions",
abstract = "The differences between pre- and postmenopausal women and men on corneocyte surface area were investigated by a noninvasive exfoliation method. Surface corneocytes were collected with a modified detergent scrub technique. Separated corneocytes were analyzed by videomicroscopy and image analyses (NIH Image™ 1.59). Additionally transepidermal water loss (TEWL) and stratum corneum hydration, water-holding capacity and moisture accumulation velocity were measured. 21 postmenopausal females without hormonal substitution (age 50.6 years), 33 premenopausal women (age 41.0 years) and 25 men matched for age (age 44.0 years) were included in this study. The smallest corneocyte surface area was seen in premenopausal women (368.0 pixels). For postmenopausal women and the male control population almost equal values could be detected (postmenopausal women 401.1 pixels; men 401.8 pixels). Significant differences were calculated with the ANOVA test (p = 0.0050) and post-hoc analyses (Dunn test); premenopausal vs. postmenopausal women (p <0.05) and premenopausal women vs. men (p <0.05), but not postmenopausal women vs. men (p > 0.05). No statistically significant differences could be detected for TEWL, stratum corneum hydration parameters. No correlation could be found between the corneocyte surface area and barrier or hydration parameters. The detected differences support the hypothesis that sexual hormones have an impact on corneocyte surface area, because sex hormone levels are higher in premenopausal women than in non-hormone-substituted postmenopausal women or men.",
keywords = "Barrier function, Corneocyte surface area, Hydration parameters, Menopause, Sex hormones, Stratum corneum, Videomicroscopy",
author = "Fluhr, {Joachim W.} and A. Pelosi and S. Lazzerini and S. Dikstein and E. Berardesca",
year = "2001",
doi = "10.1159/000056384",
language = "English",
volume = "14",
pages = "10--16",
journal = "Skin Pharmacology and Applied Skin Physiology",
issn = "1422-2868",
publisher = "S. Karger AG",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Differences in corneocyte surface area in pre- and post-menopausal women

T2 - Assessment with the noninvasive Videomicroscopic Imaging of Corneocytes Method (VIC) under basal conditions

AU - Fluhr, Joachim W.

AU - Pelosi, A.

AU - Lazzerini, S.

AU - Dikstein, S.

AU - Berardesca, E.

PY - 2001

Y1 - 2001

N2 - The differences between pre- and postmenopausal women and men on corneocyte surface area were investigated by a noninvasive exfoliation method. Surface corneocytes were collected with a modified detergent scrub technique. Separated corneocytes were analyzed by videomicroscopy and image analyses (NIH Image™ 1.59). Additionally transepidermal water loss (TEWL) and stratum corneum hydration, water-holding capacity and moisture accumulation velocity were measured. 21 postmenopausal females without hormonal substitution (age 50.6 years), 33 premenopausal women (age 41.0 years) and 25 men matched for age (age 44.0 years) were included in this study. The smallest corneocyte surface area was seen in premenopausal women (368.0 pixels). For postmenopausal women and the male control population almost equal values could be detected (postmenopausal women 401.1 pixels; men 401.8 pixels). Significant differences were calculated with the ANOVA test (p = 0.0050) and post-hoc analyses (Dunn test); premenopausal vs. postmenopausal women (p <0.05) and premenopausal women vs. men (p <0.05), but not postmenopausal women vs. men (p > 0.05). No statistically significant differences could be detected for TEWL, stratum corneum hydration parameters. No correlation could be found between the corneocyte surface area and barrier or hydration parameters. The detected differences support the hypothesis that sexual hormones have an impact on corneocyte surface area, because sex hormone levels are higher in premenopausal women than in non-hormone-substituted postmenopausal women or men.

AB - The differences between pre- and postmenopausal women and men on corneocyte surface area were investigated by a noninvasive exfoliation method. Surface corneocytes were collected with a modified detergent scrub technique. Separated corneocytes were analyzed by videomicroscopy and image analyses (NIH Image™ 1.59). Additionally transepidermal water loss (TEWL) and stratum corneum hydration, water-holding capacity and moisture accumulation velocity were measured. 21 postmenopausal females without hormonal substitution (age 50.6 years), 33 premenopausal women (age 41.0 years) and 25 men matched for age (age 44.0 years) were included in this study. The smallest corneocyte surface area was seen in premenopausal women (368.0 pixels). For postmenopausal women and the male control population almost equal values could be detected (postmenopausal women 401.1 pixels; men 401.8 pixels). Significant differences were calculated with the ANOVA test (p = 0.0050) and post-hoc analyses (Dunn test); premenopausal vs. postmenopausal women (p <0.05) and premenopausal women vs. men (p <0.05), but not postmenopausal women vs. men (p > 0.05). No statistically significant differences could be detected for TEWL, stratum corneum hydration parameters. No correlation could be found between the corneocyte surface area and barrier or hydration parameters. The detected differences support the hypothesis that sexual hormones have an impact on corneocyte surface area, because sex hormone levels are higher in premenopausal women than in non-hormone-substituted postmenopausal women or men.

KW - Barrier function

KW - Corneocyte surface area

KW - Hydration parameters

KW - Menopause

KW - Sex hormones

KW - Stratum corneum

KW - Videomicroscopy

UR - http://www.scopus.com/inward/record.url?scp=0035735485&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035735485&partnerID=8YFLogxK

U2 - 10.1159/000056384

DO - 10.1159/000056384

M3 - Article

C2 - 11509901

AN - SCOPUS:0035735485

VL - 14

SP - 10

EP - 16

JO - Skin Pharmacology and Applied Skin Physiology

JF - Skin Pharmacology and Applied Skin Physiology

SN - 1422-2868

IS - SUPPL. 1

ER -