Differences in Gene Expression and Cytokine Release Profiles Highlight the Heterogeneity of Distinct Subsets of Adipose Tissue-Derived Stem Cells in the Subcutaneous and Visceral Adipose Tissue in Humans

Sebastio Perrini, Romina Ficarella, Ernesto Picardi, Angelo Cignarelli, Maria Barbaro, Pasquale Nigro, Alessandro Peschechera, Orazio Palumbo, Massimo Carella, Michele De Fazio, Annalisa Natalicchio, Luigi Laviola, Graziano Pesole, Francesco Giorgino

Research output: Contribution to journalArticle

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Abstract

Differences in the inherent properties of adipose tissue-derived stem cells (ASC) may contribute to the biological specificity of the subcutaneous (Sc) and visceral (V) adipose tissue depots. In this study, three distinct subpopulations of ASC, i.e. ASCSVF, ASCBottom, and ASCCeiling, were isolated from Sc and V fat biopsies of non-obese subjects, and their gene expression and functional characteristics were investigated. Genome-wide mRNA expression profiles of ASCSVF, ASCBottom and ASCCeiling from Sc fat were significantly different as compared to their homologous subsets of V-ASCs. Furthermore, ASCSVF, ASCCeiling and ASCBottom from the same fat depot were also distinct from each other. In this respect, both principal component analysis and hierarchical clusters analysis showed that ASCCeiling and ASCSVF shared a similar pattern of closely related genes, which was highly different when compared to that of ASCBottom. However, larger variations in gene expression were found in inter-depot than in intra-depot comparisons. The analysis of connectivity of genes differently expressed in each ASC subset demonstrated that, although there was some overlap, there was also a clear distinction between each Sc-ASC and their corresponding V-ASC subsets, and among ASCSVF, ASCBottom, and ASCCeiling of Sc or V fat depots in regard to networks associated with regulation of cell cycle, cell organization and development, inflammation and metabolic responses. Finally, the release of several cytokines and growth factors in the ASC cultured medium also showed both inter- and intra-depot differences. Thus, ASCCeiling and ASCBottom can be identified as two genetically and functionally heterogeneous ASC populations in addition to the ASCSVF, with ASCBottom showing the highest degree of unmatched gene expression. On the other hand, inter-depot seem to prevail over intra-depot differences in the ASC gene expression assets and network functions, contributing to the high degree of specificity of Sc and V adipose tissue in humans.

Original languageEnglish
Article numbere57892
JournalPLoS One
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 5 2013

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Intra-Abdominal Fat
Subcutaneous Fat
Stem cells
Gene expression
adipose tissue
stem cells
Adipose Tissue
cytokines
Stem Cells
Fats
Tissue
Cytokines
Gene Expression
gene expression
Genes
lipids
Biopsy
Gene Regulatory Networks
subcutaneous fat
Cluster analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Differences in Gene Expression and Cytokine Release Profiles Highlight the Heterogeneity of Distinct Subsets of Adipose Tissue-Derived Stem Cells in the Subcutaneous and Visceral Adipose Tissue in Humans. / Perrini, Sebastio; Ficarella, Romina; Picardi, Ernesto; Cignarelli, Angelo; Barbaro, Maria; Nigro, Pasquale; Peschechera, Alessandro; Palumbo, Orazio; Carella, Massimo; De Fazio, Michele; Natalicchio, Annalisa; Laviola, Luigi; Pesole, Graziano; Giorgino, Francesco.

In: PLoS One, Vol. 8, No. 3, e57892, 05.03.2013.

Research output: Contribution to journalArticle

Perrini, S, Ficarella, R, Picardi, E, Cignarelli, A, Barbaro, M, Nigro, P, Peschechera, A, Palumbo, O, Carella, M, De Fazio, M, Natalicchio, A, Laviola, L, Pesole, G & Giorgino, F 2013, 'Differences in Gene Expression and Cytokine Release Profiles Highlight the Heterogeneity of Distinct Subsets of Adipose Tissue-Derived Stem Cells in the Subcutaneous and Visceral Adipose Tissue in Humans', PLoS One, vol. 8, no. 3, e57892. https://doi.org/10.1371/journal.pone.0057892
Perrini, Sebastio ; Ficarella, Romina ; Picardi, Ernesto ; Cignarelli, Angelo ; Barbaro, Maria ; Nigro, Pasquale ; Peschechera, Alessandro ; Palumbo, Orazio ; Carella, Massimo ; De Fazio, Michele ; Natalicchio, Annalisa ; Laviola, Luigi ; Pesole, Graziano ; Giorgino, Francesco. / Differences in Gene Expression and Cytokine Release Profiles Highlight the Heterogeneity of Distinct Subsets of Adipose Tissue-Derived Stem Cells in the Subcutaneous and Visceral Adipose Tissue in Humans. In: PLoS One. 2013 ; Vol. 8, No. 3.
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abstract = "Differences in the inherent properties of adipose tissue-derived stem cells (ASC) may contribute to the biological specificity of the subcutaneous (Sc) and visceral (V) adipose tissue depots. In this study, three distinct subpopulations of ASC, i.e. ASCSVF, ASCBottom, and ASCCeiling, were isolated from Sc and V fat biopsies of non-obese subjects, and their gene expression and functional characteristics were investigated. Genome-wide mRNA expression profiles of ASCSVF, ASCBottom and ASCCeiling from Sc fat were significantly different as compared to their homologous subsets of V-ASCs. Furthermore, ASCSVF, ASCCeiling and ASCBottom from the same fat depot were also distinct from each other. In this respect, both principal component analysis and hierarchical clusters analysis showed that ASCCeiling and ASCSVF shared a similar pattern of closely related genes, which was highly different when compared to that of ASCBottom. However, larger variations in gene expression were found in inter-depot than in intra-depot comparisons. The analysis of connectivity of genes differently expressed in each ASC subset demonstrated that, although there was some overlap, there was also a clear distinction between each Sc-ASC and their corresponding V-ASC subsets, and among ASCSVF, ASCBottom, and ASCCeiling of Sc or V fat depots in regard to networks associated with regulation of cell cycle, cell organization and development, inflammation and metabolic responses. Finally, the release of several cytokines and growth factors in the ASC cultured medium also showed both inter- and intra-depot differences. Thus, ASCCeiling and ASCBottom can be identified as two genetically and functionally heterogeneous ASC populations in addition to the ASCSVF, with ASCBottom showing the highest degree of unmatched gene expression. On the other hand, inter-depot seem to prevail over intra-depot differences in the ASC gene expression assets and network functions, contributing to the high degree of specificity of Sc and V adipose tissue in humans.",
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AU - Picardi, Ernesto

AU - Cignarelli, Angelo

AU - Barbaro, Maria

AU - Nigro, Pasquale

AU - Peschechera, Alessandro

AU - Palumbo, Orazio

AU - Carella, Massimo

AU - De Fazio, Michele

AU - Natalicchio, Annalisa

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