TY - JOUR
T1 - Differences in the effect of angiotensin-converting enzyme inhibitors on the rate of endothelial cell apoptosis
T2 - In vitro and in vivo studies
AU - Ceconi, C.
AU - Francolini, G.
AU - Bastianon, D.
AU - Gitti, G. L.
AU - Comini, L.
AU - Ferrari, R.
PY - 2007/12
Y1 - 2007/12
N2 - Aim: An imbalance between endothelial apoptosis and regeneration is one of the initiating events in atherosclerosis. Angiotensin-converting enzyme (ACE) inhibition corrects the endothelial dysfunction observed in coronary artery disease, and this could be the consequence of a reduction in the rate of endothelial apoptosis. The aim of this study was to investigate the effect of different ACE inhibitors on endothelial apoptosis. Methods: We examined the effect of five ACE inhibitors (enalapril, perindopril, quinapril, ramipril, and trandolapril) on the rate of endothelial apoptosis, either in vitro in human umbilical vein endothelial cells (HUVECs), using a serum deprivation method to induce apoptosis, or in vivo in rats, inducing apoptosis via endotoxic shock with Escherichia coli lipopolysaccharides (LPS). Results: We were unable to detect any significant effect of ACE inhibition on the rate of in vitro endothelial apoptosis at concentrations ranging from 5×10-8 to 10-6 M. In contrast, chronic in vivo administration of ACE inhibitors to rats at dosages that had similar hypotensive effects reduced the rate of LPS-induced apoptosis significantly for perindopril (Pramipril≫quinapril=trandolapril= enalapril, with significant differences between perindopril and quinapril (P
AB - Aim: An imbalance between endothelial apoptosis and regeneration is one of the initiating events in atherosclerosis. Angiotensin-converting enzyme (ACE) inhibition corrects the endothelial dysfunction observed in coronary artery disease, and this could be the consequence of a reduction in the rate of endothelial apoptosis. The aim of this study was to investigate the effect of different ACE inhibitors on endothelial apoptosis. Methods: We examined the effect of five ACE inhibitors (enalapril, perindopril, quinapril, ramipril, and trandolapril) on the rate of endothelial apoptosis, either in vitro in human umbilical vein endothelial cells (HUVECs), using a serum deprivation method to induce apoptosis, or in vivo in rats, inducing apoptosis via endotoxic shock with Escherichia coli lipopolysaccharides (LPS). Results: We were unable to detect any significant effect of ACE inhibition on the rate of in vitro endothelial apoptosis at concentrations ranging from 5×10-8 to 10-6 M. In contrast, chronic in vivo administration of ACE inhibitors to rats at dosages that had similar hypotensive effects reduced the rate of LPS-induced apoptosis significantly for perindopril (Pramipril≫quinapril=trandolapril= enalapril, with significant differences between perindopril and quinapril (P
KW - ACE inhibition
KW - Endothelial apoptosis
KW - HUVEC
KW - Perindopril
KW - RAEC
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U2 - 10.1007/s10557-007-6068-5
DO - 10.1007/s10557-007-6068-5
M3 - Article
C2 - 18004652
AN - SCOPUS:36848999385
VL - 21
SP - 423
EP - 429
JO - Cardiovascular Drugs and Therapy
JF - Cardiovascular Drugs and Therapy
SN - 0920-3206
IS - 6
ER -