Differences in the in vitro growth pattern of fresh and cryopreserved granulo-monopoietic precursors

M. Aglietta, W. Piacibello, A. Stacchini, F. Sanavio, A. Novarino, G. Grazia, R. Miniero, G. Neretto, F. Gavosto

Research output: Contribution to journalArticle

Abstract

A study of the in vitro growth model of human granulo-monopoietic precursors (CFU-GM) before and after cryopreservation using both leukocyte feeder layers and GCT conditioned medium as the source of colony stimulating activity (CSA) is reported. The number of colonies produced with fresh cells was linearly related to the amount of marrow seeded with both CSA sources, whereas after cryopreservation this was true with feeder layers, and with GCT only at relatively high cell concentrations. This might indicate the production of granulopoietic stimulators on the part of a second population that is at least partly resistant to freezing. It seems more likely, however, that these results depend mainly on a sublethal damage to CFU-GM induced by freezing, thus making the cells hyporesponsive to some forms of CSA, such as those contained in GCT conditioned medium.

Original languageEnglish
Pages (from-to)486-490
Number of pages5
JournalCryobiology
Volume21
Issue number5
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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    Aglietta, M., Piacibello, W., Stacchini, A., Sanavio, F., Novarino, A., Grazia, G., Miniero, R., Neretto, G., & Gavosto, F. (1984). Differences in the in vitro growth pattern of fresh and cryopreserved granulo-monopoietic precursors. Cryobiology, 21(5), 486-490. https://doi.org/10.1016/0011-2240(84)90046-4