Abstract
The morphological features of cell undergoing programmed cell death is well known and has been widely described in a number of experimental models with a variety of apoptotic triggering agents. Despite the similar cell behaviour, underlying molecular events seem variable and only partially understood. A multiple approach appears crucial to better clarify the phenomenon. The first technique, DNA gel electrophoresis, allows the identification of fragmented DNA and has been long considered the hallmark of apoptosis. Different patterns of DNA cleavage, which can be identified by conventional or 'pulsed-field gel' electrophoresis, are presented and discussed. 'In situ' labelling methods are also described both with terminal deoxynucleotidyl transferase and DNA polymerase I, aimed at the study of the distribution of DNA cleavage areas. Flow cytometry is also proposed and different technical approaches, based on different laser utilizations, are discussed. Ultrastructural analysis, allowing the study of apoptotic cell details, is finally considered.
| Original language | English |
|---|---|
| Pages (from-to) | 227-237 |
| Number of pages | 11 |
| Journal | Scanning Microscopy |
| Volume | 10 |
| Issue number | 1 |
| Publication status | Published - 1996 |
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Keywords
- 'in situ' DNA labelling
- Apoptosis
- DNA electrophoresis
- Electron microscopy
- Flow cytometry
- Freeze-fracture
ASJC Scopus subject areas
- Instrumentation
Cite this
Different approaches to the study of apoptosis. / Falcieri, E.; Stuppia, L.; Di Baldassarre, A.; Mariani, A. R.; Cinti, C.; Columbaro, M.; Zamai, L.; Vitale, M.
In: Scanning Microscopy, Vol. 10, No. 1, 1996, p. 227-237.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different approaches to the study of apoptosis
AU - Falcieri, E.
AU - Stuppia, L.
AU - Di Baldassarre, A.
AU - Mariani, A. R.
AU - Cinti, C.
AU - Columbaro, M.
AU - Zamai, L.
AU - Vitale, M.
PY - 1996
Y1 - 1996
N2 - The morphological features of cell undergoing programmed cell death is well known and has been widely described in a number of experimental models with a variety of apoptotic triggering agents. Despite the similar cell behaviour, underlying molecular events seem variable and only partially understood. A multiple approach appears crucial to better clarify the phenomenon. The first technique, DNA gel electrophoresis, allows the identification of fragmented DNA and has been long considered the hallmark of apoptosis. Different patterns of DNA cleavage, which can be identified by conventional or 'pulsed-field gel' electrophoresis, are presented and discussed. 'In situ' labelling methods are also described both with terminal deoxynucleotidyl transferase and DNA polymerase I, aimed at the study of the distribution of DNA cleavage areas. Flow cytometry is also proposed and different technical approaches, based on different laser utilizations, are discussed. Ultrastructural analysis, allowing the study of apoptotic cell details, is finally considered.
AB - The morphological features of cell undergoing programmed cell death is well known and has been widely described in a number of experimental models with a variety of apoptotic triggering agents. Despite the similar cell behaviour, underlying molecular events seem variable and only partially understood. A multiple approach appears crucial to better clarify the phenomenon. The first technique, DNA gel electrophoresis, allows the identification of fragmented DNA and has been long considered the hallmark of apoptosis. Different patterns of DNA cleavage, which can be identified by conventional or 'pulsed-field gel' electrophoresis, are presented and discussed. 'In situ' labelling methods are also described both with terminal deoxynucleotidyl transferase and DNA polymerase I, aimed at the study of the distribution of DNA cleavage areas. Flow cytometry is also proposed and different technical approaches, based on different laser utilizations, are discussed. Ultrastructural analysis, allowing the study of apoptotic cell details, is finally considered.
KW - 'in situ' DNA labelling
KW - Apoptosis
KW - DNA electrophoresis
KW - Electron microscopy
KW - Flow cytometry
KW - Freeze-fracture
UR - http://www.scopus.com/inward/record.url?scp=0030316886&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030316886&partnerID=8YFLogxK
M3 - Article
C2 - 9813608
AN - SCOPUS:0030316886
VL - 10
SP - 227
EP - 237
JO - Scanning Microscopy
JF - Scanning Microscopy
SN - 0891-7035
IS - 1
ER -