Different chemokines are expressed in human arthritic bone biopsies: IFN-γ and IL-6 differently modulate IL-8, MCP-1 and rantes production by arthritic osteoblasts

Gina Lisignoli, Stefania Toneguzzi, Francesco Grassi, Anna Piacentini, Matilde Tschon, Sandra Cristino, Gualtiero Gualtieri, Andrea Facchini

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study we analyse chemokine expression in the remodelling of subchondral bone in arthritis patients. Trabecular bone biopsies were tested by immunohistochemistry to identify interleukin (IL)-8, GRO-α, MCP-1, RANTES, MIP-1α and MIP-1β expression. Subsequently, we evaluated by immunoassay the effect of interferon (IFN)-γ and IL-6 on chemokine production by osteoarthritis (OA), rheumatoid arthritis (RA) and post-traumatic (PT) patients' isolated osteoblasts (OB). OB constitutively produced in situ IL-8, GRO-α, MCP-1, RANTES and MIP-1α. MIP-1β was positive only in mononuclear cells. In RA many of these chemokines were also produced by mononuclear cells. IFN-γ significantly down-regulated IL-8 and up-regulated MCP-1 produced by OB from all patients tested, whereas it did not affect the other chemokines analysed. Moreover, IFN-γ reduced IL-1β-stimulated IL-8 production but significantly increased both MCP-1 and RANTES. Interestingly, IL-6 significantly downregulated IFN-γ-induced MCP-1 production, that was significantly lower in OA compared to RA patients. OB expressed chemokines both in vivo and in vitro suggesting that these cells are primary effectors in the bone capable of regulating autocrine/paracrine circuits that affect bone remodelling in these diseases.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalCytokine
Volume20
Issue number5
DOIs
Publication statusPublished - Dec 2002

Keywords

  • Bone tissue
  • Chemokine
  • Cytokine
  • Osteoblasts

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

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