Different combinations of cytokines and activating receptor stimuli are required for human natural killer cell functional diversity

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Although cytokine induced NK cell activation protocols are commonly used in many laboratories worldwide, a systematic study of the effect of different cytokines either alone or in combination on NK cell function is lacking. In this study we performed a comparative evaluation of several cytokines potentially important for NK cell stimulation, focusing particularly on IL21 because of its promising role in anti-tumor therapy. To simulate in vivo physiological condition, we evaluated cytokine stimulation in total peripheral blood mononuclear cells (PBMCs), as accessory cells are responsible for the secretion of many soluble factors and can simultaneously trigger multiple activation signals through engagement of NK cell activating receptors. We show here that NK cell responses are finely regulated by several incoming stimuli and that combinations of IL21 + IL2 or IL21 + IL15 strongly induced NK cell function. Cytokine stimulation combined with NK receptor engagement can be helpful in the dissection of NK cell responses in health and disease.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalCytokine
Volume62
Issue number1
DOIs
Publication statusPublished - Apr 2013

Fingerprint

Cytokine Receptors
Natural Killer Cells
Cytokines
Chemical activation
Interleukin-15
Dissection
Natural Killer Cell Receptors
Accessories
Interleukin-2
Tumors
Blood
Health
Blood Cells
interleukin-21
Neoplasms

Keywords

  • CD107a
  • Interferon gamma
  • Interleukin 21
  • Natural killer cell receptors
  • Tumor necrosis factor alfa

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

@article{8f9adf23b22f4413b3ac1ee1da905115,
title = "Different combinations of cytokines and activating receptor stimuli are required for human natural killer cell functional diversity",
abstract = "Although cytokine induced NK cell activation protocols are commonly used in many laboratories worldwide, a systematic study of the effect of different cytokines either alone or in combination on NK cell function is lacking. In this study we performed a comparative evaluation of several cytokines potentially important for NK cell stimulation, focusing particularly on IL21 because of its promising role in anti-tumor therapy. To simulate in vivo physiological condition, we evaluated cytokine stimulation in total peripheral blood mononuclear cells (PBMCs), as accessory cells are responsible for the secretion of many soluble factors and can simultaneously trigger multiple activation signals through engagement of NK cell activating receptors. We show here that NK cell responses are finely regulated by several incoming stimuli and that combinations of IL21 + IL2 or IL21 + IL15 strongly induced NK cell function. Cytokine stimulation combined with NK receptor engagement can be helpful in the dissection of NK cell responses in health and disease.",
keywords = "CD107a, Interferon gamma, Interleukin 21, Natural killer cell receptors, Tumor necrosis factor alfa",
author = "Stefania Varchetta and Barbara Oliviero and Domenico Mavilio and Mondelli, {Mario U.}",
year = "2013",
month = "4",
doi = "10.1016/j.cyto.2013.02.018",
language = "English",
volume = "62",
pages = "58--63",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Different combinations of cytokines and activating receptor stimuli are required for human natural killer cell functional diversity

AU - Varchetta, Stefania

AU - Oliviero, Barbara

AU - Mavilio, Domenico

AU - Mondelli, Mario U.

PY - 2013/4

Y1 - 2013/4

N2 - Although cytokine induced NK cell activation protocols are commonly used in many laboratories worldwide, a systematic study of the effect of different cytokines either alone or in combination on NK cell function is lacking. In this study we performed a comparative evaluation of several cytokines potentially important for NK cell stimulation, focusing particularly on IL21 because of its promising role in anti-tumor therapy. To simulate in vivo physiological condition, we evaluated cytokine stimulation in total peripheral blood mononuclear cells (PBMCs), as accessory cells are responsible for the secretion of many soluble factors and can simultaneously trigger multiple activation signals through engagement of NK cell activating receptors. We show here that NK cell responses are finely regulated by several incoming stimuli and that combinations of IL21 + IL2 or IL21 + IL15 strongly induced NK cell function. Cytokine stimulation combined with NK receptor engagement can be helpful in the dissection of NK cell responses in health and disease.

AB - Although cytokine induced NK cell activation protocols are commonly used in many laboratories worldwide, a systematic study of the effect of different cytokines either alone or in combination on NK cell function is lacking. In this study we performed a comparative evaluation of several cytokines potentially important for NK cell stimulation, focusing particularly on IL21 because of its promising role in anti-tumor therapy. To simulate in vivo physiological condition, we evaluated cytokine stimulation in total peripheral blood mononuclear cells (PBMCs), as accessory cells are responsible for the secretion of many soluble factors and can simultaneously trigger multiple activation signals through engagement of NK cell activating receptors. We show here that NK cell responses are finely regulated by several incoming stimuli and that combinations of IL21 + IL2 or IL21 + IL15 strongly induced NK cell function. Cytokine stimulation combined with NK receptor engagement can be helpful in the dissection of NK cell responses in health and disease.

KW - CD107a

KW - Interferon gamma

KW - Interleukin 21

KW - Natural killer cell receptors

KW - Tumor necrosis factor alfa

UR - http://www.scopus.com/inward/record.url?scp=84875803691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875803691&partnerID=8YFLogxK

U2 - 10.1016/j.cyto.2013.02.018

DO - 10.1016/j.cyto.2013.02.018

M3 - Article

C2 - 23490421

AN - SCOPUS:84875803691

VL - 62

SP - 58

EP - 63

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -