Different combinations of cytokines and activating receptor stimuli are required for human natural killer cell functional diversity

Research output: Contribution to journalArticlepeer-review

Abstract

Although cytokine induced NK cell activation protocols are commonly used in many laboratories worldwide, a systematic study of the effect of different cytokines either alone or in combination on NK cell function is lacking. In this study we performed a comparative evaluation of several cytokines potentially important for NK cell stimulation, focusing particularly on IL21 because of its promising role in anti-tumor therapy. To simulate in vivo physiological condition, we evaluated cytokine stimulation in total peripheral blood mononuclear cells (PBMCs), as accessory cells are responsible for the secretion of many soluble factors and can simultaneously trigger multiple activation signals through engagement of NK cell activating receptors. We show here that NK cell responses are finely regulated by several incoming stimuli and that combinations of IL21 + IL2 or IL21 + IL15 strongly induced NK cell function. Cytokine stimulation combined with NK receptor engagement can be helpful in the dissection of NK cell responses in health and disease.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalCytokine
Volume62
Issue number1
DOIs
Publication statusPublished - Apr 2013

Keywords

  • CD107a
  • Interferon gamma
  • Interleukin 21
  • Natural killer cell receptors
  • Tumor necrosis factor alfa

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Different combinations of cytokines and activating receptor stimuli are required for human natural killer cell functional diversity'. Together they form a unique fingerprint.

Cite this