Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells

Serena Incerti Parenti, Luigi Checchi, Milena Fini, Matilde Tschon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Because osteoblasts play a key role in bone remodeling and the influence of low-level laser therapy on this process is not clear, Saos-2 human osteoblast-like cells were irradiated by a gallium aluminum arsenide diode laser (915 nm) for 10, 48, 96, 193, and 482 s using doses 1, 5, 10, 20, and 50 J/cm2, respectively. A control group was not irradiated. Morphology, viability, and cytotoxicity analyses were carried out after 1 hr, 1 day, and 3 days. Deoxyribose nucleic acid (DNA) content and release of vascular endothelial growth factor (VEGF), receptor activator of nuclear factor kappa B ligand (RANKL), and osteoprotegerin (OPG) were evaluated. Viability was modulated by laser irradiation in a dose-dependent manner, with 10 J/cm2 inducing a biostimulatory response and 20 to 50 J/cm2 determining a bioinhibitory and cytotoxic effect. Accordingly, DNA content was generally increased for the 10 J/cm2 dose and decreased for the 50 J/cm2 dose. A rapid and transitory trend toward increased RANKL/OPG ratio and a t ndency toward a delayed increase in VEGF release for doses of 1 to 10 J/cm2 was found. Further investigations using the biostimulatory dose of 10 J/cm2 emerged from this study are needed to establish the ideal treatment regimens in the laboratory as well as in clinical practice.

Original languageEnglish
Article number108002
JournalJournal of Biomedical Optics
Volume19
Issue number10
DOIs
Publication statusPublished - Oct 1 2014

Fingerprint

RANK Ligand
Deoxyribose
Osteoprotegerin
osteoblasts
Semiconductor Lasers
Nucleic acids
Osteoblasts
Laser beam effects
Nucleic Acids
Dosimetry
Aluminum gallium arsenide
Lasers
Ligands
dosage
Vascular Endothelial Growth Factor Receptor
irradiation
Bone Remodeling
Cytotoxicity
cells
Vascular Endothelial Growth Factor A

Keywords

  • Biophysical stimulation
  • bone remodeling
  • diode laser
  • in vitro study
  • osteoblast-like cells

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Medicine(all)

Cite this

Different doses of low-level laser irradiation modulate the in vitro response of osteoblast-like cells. / Incerti Parenti, Serena; Checchi, Luigi; Fini, Milena; Tschon, Matilde.

In: Journal of Biomedical Optics, Vol. 19, No. 10, 108002, 01.10.2014.

Research output: Contribution to journalArticle

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