TY - JOUR
T1 - Different effects of high and low shear stress on platelet-derived growth factor isoform release by endothelial cells
T2 - Consequences for smooth muscle cell migration
AU - Palumbo, Roberta
AU - Gaetano, Carlo
AU - Antonini, Annalisa
AU - Pompilio, Giulio
AU - Bracco, Enrico
AU - Rönnstrand, Lars
AU - Heldin, Carl Henrik
AU - Capogrossi, Maurizio C.
PY - 2002
Y1 - 2002
N2 - In the present study, we analyzed the effect of conditioned media (CM) from bovine aortic endothelial cells exposed to laminar shear stress (SS) of 5 dyne/cm2 (SS5) or 15 dyne/cm2 (SS15) for 16 hours on smooth muscle cell (SMC) migration. In response to CM from bovine aortic endothelial cells exposed to SS5 (CMSS5) and SS15 (CMSS15), migration was 45±5.5 and 30±1.5 cells per field, respectively (P2 and also when SS of 5 and 15 dyne/cm2 lasted 24 hours. Platelet-derived growth factor (PDGF)-AA levels in CMSS5 and CMSS15 were 9±7 and 18±5 ng/106 cells for 16 hours, respectively (P6 cells for 16 hours, respectively (PCMSS5. In response to CMSS15, a neutralizing antibody against PDGF-AA enhanced SMC migration to a level comparable to that of CMSS5; in contrast, antibodies against PDGF-BB abolished SMC migration. Transfection of SMCs with a dominant-negative PDGFRα or PDGFRβ increased or inhibited, respectively, SMC migration in response to CMSS15. Overexpression of wild-type PDGFRα inhibited SMC migration in response to CMSS5, CMSS15, or recombinant PDGF-BB (P
AB - In the present study, we analyzed the effect of conditioned media (CM) from bovine aortic endothelial cells exposed to laminar shear stress (SS) of 5 dyne/cm2 (SS5) or 15 dyne/cm2 (SS15) for 16 hours on smooth muscle cell (SMC) migration. In response to CM from bovine aortic endothelial cells exposed to SS5 (CMSS5) and SS15 (CMSS15), migration was 45±5.5 and 30±1.5 cells per field, respectively (P2 and also when SS of 5 and 15 dyne/cm2 lasted 24 hours. Platelet-derived growth factor (PDGF)-AA levels in CMSS5 and CMSS15 were 9±7 and 18±5 ng/106 cells for 16 hours, respectively (P6 cells for 16 hours, respectively (PCMSS5. In response to CMSS15, a neutralizing antibody against PDGF-AA enhanced SMC migration to a level comparable to that of CMSS5; in contrast, antibodies against PDGF-BB abolished SMC migration. Transfection of SMCs with a dominant-negative PDGFRα or PDGFRβ increased or inhibited, respectively, SMC migration in response to CMSS15. Overexpression of wild-type PDGFRα inhibited SMC migration in response to CMSS5, CMSS15, or recombinant PDGF-BB (P
KW - Endothelial cells
KW - Platelet-derived growth factor receptors
KW - Platelet-derived growth factors
KW - Shear stress
KW - Smooth muscle cells
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U2 - 10.1161/hq0302.104528
DO - 10.1161/hq0302.104528
M3 - Article
C2 - 11884282
AN - SCOPUS:0036123021
VL - 22
SP - 405
EP - 411
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 3
ER -