TY - JOUR
T1 - Different expression of β-N-acetylhexosaminidase in mouse tissues
AU - Della Fazia, M. A.
AU - Beccari, T.
AU - Servillo, G.
AU - Viola-Magni, M. P.
AU - Orlacchio, A.
PY - 1994
Y1 - 1994
N2 - The expression of genes encoding for the α and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase was investigated in different mouse tissues. It was found, using fluorogenic substrates, that the amounts of α and β subunits were not the same in different tissues: α-subunit was more abundant in the brain, β-subunit in epididymis and brain. The different isoenzyme patterns and specific activities in mouse tissues are due to the differences in the amount of hexosaminidase subunits. The mRNA, evaluated by Northern blotting analyses, revealed a greater expression of α-subunit in the testis and of β-subunit in the brain and epididymis. The results indicate, therefore, that gene expression and the amount of subunits are in good relationship for β-subunit, whereas there is no correlation for α-subunit.
AB - The expression of genes encoding for the α and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase was investigated in different mouse tissues. It was found, using fluorogenic substrates, that the amounts of α and β subunits were not the same in different tissues: α-subunit was more abundant in the brain, β-subunit in epididymis and brain. The different isoenzyme patterns and specific activities in mouse tissues are due to the differences in the amount of hexosaminidase subunits. The mRNA, evaluated by Northern blotting analyses, revealed a greater expression of α-subunit in the testis and of β-subunit in the brain and epididymis. The results indicate, therefore, that gene expression and the amount of subunits are in good relationship for β-subunit, whereas there is no correlation for α-subunit.
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U2 - 10.1006/bbrc.1994.1378
DO - 10.1006/bbrc.1994.1378
M3 - Article
C2 - 8147878
AN - SCOPUS:0028174942
VL - 199
SP - 1341
EP - 1346
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -