TY - JOUR
T1 - Different expression of CD44, ICAM-1, and HSP60 on primary tumor and metastases of a human pancreatic carcinoma growing in scid mice
AU - Piselli, Pierluca
AU - Vendetti, Silvia
AU - Vismara, Daniela
AU - Cicconi, Rosella
AU - Poccia, Fabrizio
AU - Colizzi, Vittorio
AU - Delplno, Andrea
PY - 2000
Y1 - 2000
N2 - Surface adhesion molecules play an important, but still not completely clarified role in tumor metastasization. In this research, FAGS analysis was employed to analyze surface expression of CD44H, CD44v5, CD44v6, ICAM-1 and HSP60 in human pancreatic adenocarcinoma cells growing in vitro or collected ex vivo from primary tumors and lung metastases of tumor-engrafted SCID mice. It was found that, in metastatic cells, the standard form of CD44 (CD44H) is down,-regulated, while a large fraction of cells express on membrane the splice variants v5/v6 and, in addition, ICAM-1 and HSP60. It was also apparent that two cell populations are present in lung metastases: a CD44(neg) population, including cells expressing CD44v5/v6, ICAM-1 and HSP60 and a population of CD44(pos), CD44v5/v6(neg), ICAM-1(neg) and HSP6(neg) cells. These results demonstrate that, in pancreatic adenocarcinomas, metastasization is correlated with expression of the CD44 variants v5 and v6. Moreover, this is the first report demonstrating HSP60 surface expression on metastatic cells.
AB - Surface adhesion molecules play an important, but still not completely clarified role in tumor metastasization. In this research, FAGS analysis was employed to analyze surface expression of CD44H, CD44v5, CD44v6, ICAM-1 and HSP60 in human pancreatic adenocarcinoma cells growing in vitro or collected ex vivo from primary tumors and lung metastases of tumor-engrafted SCID mice. It was found that, in metastatic cells, the standard form of CD44 (CD44H) is down,-regulated, while a large fraction of cells express on membrane the splice variants v5/v6 and, in addition, ICAM-1 and HSP60. It was also apparent that two cell populations are present in lung metastases: a CD44(neg) population, including cells expressing CD44v5/v6, ICAM-1 and HSP60 and a population of CD44(pos), CD44v5/v6(neg), ICAM-1(neg) and HSP6(neg) cells. These results demonstrate that, in pancreatic adenocarcinomas, metastasization is correlated with expression of the CD44 variants v5 and v6. Moreover, this is the first report demonstrating HSP60 surface expression on metastatic cells.
KW - CD44
KW - HSP60
KW - ICAM-1
KW - Metastasis
KW - Panreatic carcinoma
UR - http://www.scopus.com/inward/record.url?scp=0034009727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034009727&partnerID=8YFLogxK
M3 - Article
C2 - 10810361
AN - SCOPUS:0034009727
VL - 20
SP - 825
EP - 831
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 2 A
ER -