Different genome organization in two new cell lines established from human gastric carcinoma

Livia Bertoni, Wainer Zoli, Elena Mucciolo, Luca Ricotti, Solomon Nergadze, Dino Amadori, Elena Giulotto

Research output: Contribution to journalArticlepeer-review

Abstract

Two gastric cancer cell lines, AKG and GK2, were established from a pleural and an ascitic effusion, respectively. GK2 cells have a pseudodiploid karyotype with an add(6)(q27) chromosome in all metaphases examined. The karyotype of AKG cells is highly rearranged: FISH analysis with painting probes has shown that DNA sequences derived from single chromosomes are scattered on several (as many as eight) markers. In this cell line, the C- MYC and the K-RAS oncogenes are amplified. The organization and the copy number of the C-MYC-amplified units are different from the K-RAS units, suggesting that the two oncogenes were amplified independently. The presence of a few marker chromosomes carrying both C-MYC and K-RAS could be due to translocation events that followed the amplification.

Original languageEnglish
Pages (from-to)152-159
Number of pages8
JournalCancer Genetics and Cytogenetics
Volume105
Issue number2
DOIs
Publication statusPublished - Sep 1998

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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