Different genome organization in two new cell lines established from human gastric carcinoma

Livia Bertoni, Wainer Zoli, Elena Mucciolo, Luca Ricotti, Solomon Nergadze, Dino Amadori, Elena Giulotto

Research output: Contribution to journalArticle

Abstract

Two gastric cancer cell lines, AKG and GK2, were established from a pleural and an ascitic effusion, respectively. GK2 cells have a pseudodiploid karyotype with an add(6)(q27) chromosome in all metaphases examined. The karyotype of AKG cells is highly rearranged: FISH analysis with painting probes has shown that DNA sequences derived from single chromosomes are scattered on several (as many as eight) markers. In this cell line, the C- MYC and the K-RAS oncogenes are amplified. The organization and the copy number of the C-MYC-amplified units are different from the K-RAS units, suggesting that the two oncogenes were amplified independently. The presence of a few marker chromosomes carrying both C-MYC and K-RAS could be due to translocation events that followed the amplification.

Original languageEnglish
Pages (from-to)152-159
Number of pages8
JournalCancer Genetics and Cytogenetics
Volume105
Issue number2
DOIs
Publication statusPublished - Sep 1998

Fingerprint

Karyotype
Oncogenes
Stomach
Genome
Carcinoma
Cell Line
Paintings
Chromosomes, Human, Pair 6
Pleural Effusion
Metaphase
Genetic Markers
Stomach Neoplasms
Chromosomes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Bertoni, L., Zoli, W., Mucciolo, E., Ricotti, L., Nergadze, S., Amadori, D., & Giulotto, E. (1998). Different genome organization in two new cell lines established from human gastric carcinoma. Cancer Genetics and Cytogenetics, 105(2), 152-159. https://doi.org/10.1016/S0165-4608(98)00023-5

Different genome organization in two new cell lines established from human gastric carcinoma. / Bertoni, Livia; Zoli, Wainer; Mucciolo, Elena; Ricotti, Luca; Nergadze, Solomon; Amadori, Dino; Giulotto, Elena.

In: Cancer Genetics and Cytogenetics, Vol. 105, No. 2, 09.1998, p. 152-159.

Research output: Contribution to journalArticle

Bertoni, L, Zoli, W, Mucciolo, E, Ricotti, L, Nergadze, S, Amadori, D & Giulotto, E 1998, 'Different genome organization in two new cell lines established from human gastric carcinoma', Cancer Genetics and Cytogenetics, vol. 105, no. 2, pp. 152-159. https://doi.org/10.1016/S0165-4608(98)00023-5
Bertoni, Livia ; Zoli, Wainer ; Mucciolo, Elena ; Ricotti, Luca ; Nergadze, Solomon ; Amadori, Dino ; Giulotto, Elena. / Different genome organization in two new cell lines established from human gastric carcinoma. In: Cancer Genetics and Cytogenetics. 1998 ; Vol. 105, No. 2. pp. 152-159.
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