Different initiation of pre-TCR and γδTCR signalling

Claude Saint-Ruf, Maddalena Panigada, Orly Azogul, Pascale Debey, Harald Von Boehmer, Fabio Grassi

Research output: Contribution to journalArticle

Abstract

Lineage choice is of great interest in developmental biology. In the immune system, the αβ and γδ lineages of Tlymphocytes diverge during the course of the β-, γ- and δ-chain rearrangement of T-cell receptor (TCR) genes that takes place within the same precursor cell and which results in the formation of the γδTCR or pre-TCR proteins. The pre-TCR consists of the TCRβ chain covalently linked to the pre-TCRα protein, which is present in immature but not in mature T cells which instead express the TCRα chain. Animals deficient in pre-TCRα have few αβ lineage cells but an increased number of γδ T cells. These γδT cells exhibit more extensive TCRβ rearrangement than γδ T cells from wild-type mice. These observations are consistent with the idea that different signals emanating from the γδTCR and pre-TCR instruct lineage commitment. Here we show, by using confocal microscopy and biochemistry to analyse the initiation of signalling, that the pre-TCR but not the γδTCR colocalizes with the p56(lck) Src kinase into glycolipid-enriched membrane domains (rafts) apparently without any need for ligation. This results in the phosphorylation of CD3ε and Zap-70 signal transducing molecules. The results indicate clear differences between pre-TCR and γδTCR signalling.

Original languageEnglish
Pages (from-to)524-527
Number of pages4
JournalNature
Volume406
Issue number6795
DOIs
Publication statusPublished - Aug 3 2000

ASJC Scopus subject areas

  • General

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    Saint-Ruf, C., Panigada, M., Azogul, O., Debey, P., Von Boehmer, H., & Grassi, F. (2000). Different initiation of pre-TCR and γδTCR signalling. Nature, 406(6795), 524-527. https://doi.org/10.1038/35020093