Different mechanisms of ‘in vitro’ lymphocytotoxicity against isolated rabbit hepatocytes during the course of acute viral hepatitis

G. F. Stefanini, M. Bernardi, F. Miglio, E. Mariani, F. Chiodo, A. Facchini, G. Labò, G. Gasbarrini

Research output: Contribution to journalArticle

Abstract

In order to characterize the effector mechanism of ‘in vitro’ lymphocytotoxicity against isolated rabbit liver cells in acute viral hepatitis (AVH). the test was performed in 4 patients with type A, in 8 with type B. and in 2 patients with non-A non-B AVH: (a) using peripheral blood lymphocytes; (b) after enzymatic digestion of the surface membrane immunoglobulins of these lymphocytes, and (c) as in b with addition of patients’ sera. 78.5% of the patients had an elevated cytotoxicity which did not change when the lymphocytes were treated as in b and c. Only the 2 patients which progressed to chronicity had an elevated cytotoxicity which disappeared after enzymatic exposure and reappeared with the addition of patients’ sera. The results suggest that ‘in vitro’ cytotoxicity in recovering AVH is not antibody dependent, but probably of spontaneous type. On the contrary an antibodydependent cytotoxicity is present in the 2 cases of AVH who progressed to chronicity.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalDigestion
Volume22
Issue number5
DOIs
Publication statusPublished - 1981

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Keywords

  • Acute viral hepatitis
  • Antibody-dependent cellmediated cytotoxicity
  • Chronic active hepatitis
  • K lymphocytes
  • NK lymphocytes
  • Spontaneous cell-mediated cytotoxicity

ASJC Scopus subject areas

  • Gastroenterology

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