Abstract
Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P
| Original language | English |
|---|---|
| Pages (from-to) | 1673-1679 |
| Number of pages | 7 |
| Journal | Journal of Pediatric Surgery |
| Volume | 39 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 2004 |
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Keywords
- alveolar
- immunohistochemistry
- in situ zymography
- Matrix metalloproteinases
- rhabdomyosarcoma
ASJC Scopus subject areas
- Surgery
Cite this
Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma. / Diomedi-Camassei, Francesca; Boldrini, Renata; Ravà, Lucilla; Donfrancesco, Alberto; Boglino, Camillo; Messina, Elisa; Dominici, Carlo; Callea, Francesco.
In: Journal of Pediatric Surgery, Vol. 39, No. 11, 11.2004, p. 1673-1679.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma
AU - Diomedi-Camassei, Francesca
AU - Boldrini, Renata
AU - Ravà, Lucilla
AU - Donfrancesco, Alberto
AU - Boglino, Camillo
AU - Messina, Elisa
AU - Dominici, Carlo
AU - Callea, Francesco
PY - 2004/11
Y1 - 2004/11
N2 - Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P
AB - Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P
KW - alveolar
KW - immunohistochemistry
KW - in situ zymography
KW - Matrix metalloproteinases
KW - rhabdomyosarcoma
UR - http://www.scopus.com/inward/record.url?scp=8444239278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=8444239278&partnerID=8YFLogxK
U2 - 10.1016/j.jpedsurg.2004.07.014
DO - 10.1016/j.jpedsurg.2004.07.014
M3 - Article
C2 - 15547833
AN - SCOPUS:8444239278
VL - 39
SP - 1673
EP - 1679
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
SN - 0022-3468
IS - 11
ER -