Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma

Francesca Diomedi-Camassei; Renata Boldrini; Lucilla Ravà; Alberto Donfrancesco; Camillo Boglino; Elisa Messina; Carlo Dominici; Francesco Callea

doi:10.1016/j.jpedsurg.2004.07.014

Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma

Francesca Diomedi-Camassei, Renata Boldrini, Lucilla Ravà, Alberto Donfrancesco, Camillo Boglino, Elisa Messina, Carlo Dominici, Francesco Callea

Ospedale Pediatrico Bambino Gesu

Research output: Contribution to journal › Article › peer-review

Abstract

Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P

Original language	English
Pages (from-to)	1673-1679
Number of pages	7
Journal	Journal of Pediatric Surgery
Volume	39
Issue number	11
DOIs	https://doi.org/10.1016/j.jpedsurg.2004.07.014
Publication status	Published - Nov 2004

Keywords

alveolar
immunohistochemistry
in situ zymography
Matrix metalloproteinases
rhabdomyosarcoma

ASJC Scopus subject areas

Surgery

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10.1016/j.jpedsurg.2004.07.014

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Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma. / Diomedi-Camassei, Francesca; Boldrini, Renata ; Ravà, Lucilla; Donfrancesco, Alberto; Boglino, Camillo; Messina, Elisa; Dominici, Carlo; Callea, Francesco.

In: Journal of Pediatric Surgery, Vol. 39, No. 11, 11.2004, p. 1673-1679.

Research output: Contribution to journal › Article › peer-review

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abstract = "Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P ",

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author = "Francesca Diomedi-Camassei and Renata Boldrini and Lucilla Rav{\`a} and Alberto Donfrancesco and Camillo Boglino and Elisa Messina and Carlo Dominici and Francesco Callea",

year = "2004",

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AU - Diomedi-Camassei, Francesca

AU - Boldrini, Renata

AU - Ravà, Lucilla

AU - Donfrancesco, Alberto

AU - Boglino, Camillo

AU - Messina, Elisa

AU - Dominici, Carlo

AU - Callea, Francesco

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N2 - Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P

AB - Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P

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