Different pattern of matrix metalloproteinases expression in alveolar versus embryonal rhabdomyosarcoma

Francesca Diomedi-Camassei, Renata Boldrini, Lucilla Ravà, Alberto Donfrancesco, Camillo Boglino, Elisa Messina, Carlo Dominici, Francesco Callea

Research output: Contribution to journalArticlepeer-review


Background/purpose The matrix metalloproteinases (MMPs), a family of enzymes that degrade the extracellular matrix (ECM), are important in neoplastic cell invasion and metastasis. Data for rhabdomyosarcoma (RMS), the most frequent soft tissue sarcoma of childhood, are lacking. The aim of this study was to assess their expression in this tumor and to evaluate the correlation with clinicopathologic parameters. Methods Immunohistochemical expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, TIMP-1, and TIMP-2 was investigated in 33 human RMSs, 12 alveolar, and 21 embryonal histologic subtypes (12 high risk and 9 low/standard risk). Evaluation of the results was based on the percent of positive neoplastic cells and on staining intensity (negative, moderate, and strong). In situ zymography was carried out on 4 frozen RMS samples (2 alveolar and 2 high-risk embryonal). Results Alveolar type showed a stronger MMP-1, -2 and -9 expression in comparison with embryonal (P = .006, P

Original languageEnglish
Pages (from-to)1673-1679
Number of pages7
JournalJournal of Pediatric Surgery
Issue number11
Publication statusPublished - Nov 2004


  • alveolar
  • immunohistochemistry
  • in situ zymography
  • Matrix metalloproteinases
  • rhabdomyosarcoma

ASJC Scopus subject areas

  • Surgery


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