Different patterns of neuronal activation and neurodegeneration in the thalamus and cortex of epilepsy-resistant Proechimys rats versus Wistar rats after pilocarpine-induced protracted seizures

Anna Andrioli, Paolo F. Fabene, Roberto Spreafico, Esper A. Cavalheiro, Marina Bentivoglio

Research output: Contribution to journalArticle

Abstract

Purpose: To analyze cellular mechanisms of limbic-seizure suppression, the response to pilocarpine-induced seizures was investigated in cortex and thalamus, comparing epilepsy-resistant rats Proechimys guyannensis with Wistar rats. Methods: Fos immunoreactivity revealing neuronal activation, and degenerating neurons labeled by Fluoro-Jade B (FJB) histochemistry were analyzed on the first day after onset of seizures lasting 3 h. Subpopulations of γ-aminobutyric acid (GABA)ergic cells were characterized with double Fos-parvalbumin immunohistochemistry. Results: In both cortex and thalamus, degenerating neurons were much fewer in Proechimys than Wistar rats. Fos persisted at high levels at 24 h only in the Proechimys thalamus and cortex, especially in layer VI where corticothalamic neurons reside. In the parietal cortex, about 50% of parvalbumin-containing interneurons at 8 h, and 10-20% at 24 h, were Fos-positive in Wistar rats, but in Proechimys, Fos was expressed in almost all parvalbumin-containing interneurons at 8 h and dropped at 24 h. Fos positivity in cingulate cortex interneurons was similar in both species. In the Wistar rat thalamus, Fos was induced in medial and midline nuclei up to 8 h, when

Original languageEnglish
Pages (from-to)832-848
Number of pages17
JournalEpilepsia
Volume50
Issue number4
DOIs
Publication statusPublished - Apr 2009

Keywords

  • Cell death
  • Cingulate cortex
  • Corticothalamic system
  • Fos
  • Neocortex
  • Thalamic reticular nucleus

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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