Different quantitative apoptotic traits in coronary atherosclerotic plaques from patients with stable angina pectoris and acute coronary syndromes

Marco L. Rossi, Nicola Marziliano, Piera Angelica Merlini, Ezio Bramucci, Umberto Canosi, Guido Belli, Dennis Zavalloni Parenti, Pier Mannuccio Mannucci, Diego Ardissino

Research output: Contribution to journalArticlepeer-review

Abstract

Background - Apoptosis in human atherosclerotic coronary plaques possibly causes plaque destabilization by contributing to the weakening and breaking down of the fibrous cap. We tested the hypothesis that apoptosis is quantitatively increased in unstable atherosclerotic plaques. Methods and Results - We analyzed the expression of apoptotic genes such as BAX, CASP1, FAS, FAS L, FOS, MDM2, NFkB2, P53, PCNA, TERT, and XRCC1 in coronary plaques collected with directional coronary atherectomy from 15 patients with stable angina and 15 with acute coronary syndromes without ST elevation (ACS). Total RNA was extracted and cDNA was amplified with a specific set of primers and TaqMan probes. Apoptosis was also revealed by DNA laddering. To clarify the source of mRNAs, we performed in situ reverse transcriptase-polymerase chain reaction coupled with immunocytochemistry and found a substantial overlap between the mRNAs of the above genes and vascular smooth muscle cells. Gene expression analysis showed that the proapoptotic genes (ie, BAX, CASP1, FAS, FAS L, FOS, NFkB2, P53, PCNA) were significantly more expressed (P

Original languageEnglish
Pages (from-to)1767-1773
Number of pages7
JournalCirculation
Volume110
Issue number13
DOIs
Publication statusPublished - Sep 28 2004

Keywords

  • Acute coronary syndromes
  • Angina
  • Apoptosis
  • Atherosclerosis
  • Gene expression

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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