Different rates of CD4+ and CD8+ T-cell proliferation in interleukin-2 - Treated human immunodeficiency virus-positive subjects

L. Caggiari, S. Zanussi, C. Crepaldi, M. T. Bortolin, C. Caffau, M. D'Andrea, P. De Paoli

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Interleukin-2 (IL-2) has been used successfully to increase CD4 cell counts in patients who are human immunodeficiency virus (HIV) positive. The mechanisms involved in this phenomenon are unknown. We hypothesized that a differential proliferation rate of CD4+ compared with CD8+ lymphocytes could be related to the increase of CD4 counts and of CD4/CD8 ratios that occur in HIV+ patients during IL-2 treatment. Methods: We enrolled in our study 14 HIV+ patients treated with IL-2 or with highly active antiretroviral therapy (HAART) during a 96-week observation period. Using flow cytometry, we measured longitudinally the expression of the Ki67 antigen in peripheral blood CD4+ and CD8+ lymphocyte subsets. Results: Compared with HAART alone, IL-2 produced a rapid increase of Ki67+ proliferating CD4 cells and a concomitant increase of the CD4/CD8 ratios, whereas the corresponding CD8 proliferation increased slightly. On the contrary, HAART alone was effective in suppressing equally both CD4 and CD8 proliferation. Conclusions: Our results suggest a selective activity of IL-2 on CD4 T-cell proliferation; on the contrary, CD8-specific proliferation is affected minimally during treatment. This information may offer the potential to plan correctly immune activating regimens.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalCytometry
Volume44
Issue number4
Publication statusPublished - Aug 15 2001

Keywords

  • CD4
  • CD8
  • HIV
  • IL-2 therapy
  • Ki67

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

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