Different Sialoside Epitopes on Collagen Film Surfaces Direct Mesenchymal Stem Cell Fate

Antonella Sgambato, Laura Russo, Monica Montesi, Silvia Panseri, Maurilio Marcacci, Elena Caravà, Mario Raspanti, Laura Cipolla

Research output: Contribution to journalReview articlepeer-review

Abstract

3′-Sialyllactose and 6′-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes. In particular, 3′-sialyllactose significantly upregulate the expression of RUNX2 and ALP, well-known markers of osteogenesis, whereas 6′-sialyllactose up-regulate the expression of chondrocyte marker ACAN. Because no osteogenic or chondrogenic supplements in culture media were added, the inductive effect in terms of increased gene expression has to be ascribed uniquely to collagen surface functionalization. These results support the promising role of sialosides in the regulation of stem cells fate and open brilliant perspective for the future use of the presented approach toward osteochondral tissue engineering applications.

Original languageEnglish
Pages (from-to)14952-14957
Number of pages6
JournalACS Applied Materials and Interfaces
Volume8
Issue number24
DOIs
Publication statusPublished - Jun 22 2016

Keywords

  • carbohydrates
  • chondrogenesis
  • collagen
  • mesenchymal stem cells
  • osteogenesis
  • sialic acid

ASJC Scopus subject areas

  • Materials Science(all)

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