Abstract
3′-Sialyllactose and 6′-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes. In particular, 3′-sialyllactose significantly upregulate the expression of RUNX2 and ALP, well-known markers of osteogenesis, whereas 6′-sialyllactose up-regulate the expression of chondrocyte marker ACAN. Because no osteogenic or chondrogenic supplements in culture media were added, the inductive effect in terms of increased gene expression has to be ascribed uniquely to collagen surface functionalization. These results support the promising role of sialosides in the regulation of stem cells fate and open brilliant perspective for the future use of the presented approach toward osteochondral tissue engineering applications.
| Original language | English |
|---|---|
| Pages (from-to) | 14952-14957 |
| Number of pages | 6 |
| Journal | ACS Applied Materials and Interfaces |
| Volume | 8 |
| Issue number | 24 |
| DOIs | |
| Publication status | Published - Jun 22 2016 |
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Keywords
- carbohydrates
- chondrogenesis
- collagen
- mesenchymal stem cells
- osteogenesis
- sialic acid
ASJC Scopus subject areas
- Materials Science(all)
Cite this
Different Sialoside Epitopes on Collagen Film Surfaces Direct Mesenchymal Stem Cell Fate. / Sgambato, Antonella; Russo, Laura; Montesi, Monica; Panseri, Silvia; Marcacci, Maurilio; Caravà, Elena; Raspanti, Mario; Cipolla, Laura.
In: ACS Applied Materials and Interfaces, Vol. 8, No. 24, 22.06.2016, p. 14952-14957.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Different Sialoside Epitopes on Collagen Film Surfaces Direct Mesenchymal Stem Cell Fate
AU - Sgambato, Antonella
AU - Russo, Laura
AU - Montesi, Monica
AU - Panseri, Silvia
AU - Marcacci, Maurilio
AU - Caravà, Elena
AU - Raspanti, Mario
AU - Cipolla, Laura
PY - 2016/6/22
Y1 - 2016/6/22
N2 - 3′-Sialyllactose and 6′-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes. In particular, 3′-sialyllactose significantly upregulate the expression of RUNX2 and ALP, well-known markers of osteogenesis, whereas 6′-sialyllactose up-regulate the expression of chondrocyte marker ACAN. Because no osteogenic or chondrogenic supplements in culture media were added, the inductive effect in terms of increased gene expression has to be ascribed uniquely to collagen surface functionalization. These results support the promising role of sialosides in the regulation of stem cells fate and open brilliant perspective for the future use of the presented approach toward osteochondral tissue engineering applications.
AB - 3′-Sialyllactose and 6′-sialyllactose have been covalently linked to collagen films. Preliminary in vitro study on the behavior of mesenchymal stem cells (MSCs) in terms of cell viability, proliferation and induction of osteogenic and chondrogenic related genes has been performed. Results indicate that sialoside epitopes on collagen surface represent a suitable support for MSCs adhesion and cell proliferation, moreover, the neoglycosylation provide MSCs with different and specific stimuli, saccharide-type depending, in term of expression of osteogenic and chondrogenic related genes. In particular, 3′-sialyllactose significantly upregulate the expression of RUNX2 and ALP, well-known markers of osteogenesis, whereas 6′-sialyllactose up-regulate the expression of chondrocyte marker ACAN. Because no osteogenic or chondrogenic supplements in culture media were added, the inductive effect in terms of increased gene expression has to be ascribed uniquely to collagen surface functionalization. These results support the promising role of sialosides in the regulation of stem cells fate and open brilliant perspective for the future use of the presented approach toward osteochondral tissue engineering applications.
KW - carbohydrates
KW - chondrogenesis
KW - collagen
KW - mesenchymal stem cells
KW - osteogenesis
KW - sialic acid
UR - http://www.scopus.com/inward/record.url?scp=84975796448&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975796448&partnerID=8YFLogxK
U2 - 10.1021/acsami.5b08270
DO - 10.1021/acsami.5b08270
M3 - Review article
VL - 8
SP - 14952
EP - 14957
JO - ACS applied materials & interfaces
JF - ACS applied materials & interfaces
SN - 1944-8244
IS - 24
ER -