Different subcellular localization of ALCAM molecules in neuroblastoma: Association with relapse

Maria Valeria Corrias, Claudio Gambini, Andrea Gregorio, Michela Croce, Gaia Barisione, Claudia Cossu, Armando Rossello, Silvano Ferrini, Marina Fabbi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166), involved in nervous system development, has been linked to tumor progression and metastasis in several tumors. No information is available on ALCAM expression in neuroblastoma, a childhood neoplasia originating from the sympathetic nervous system. Methods: ALCAM expression was analysed by immunofluorescence and immunohistochemistry on differentiated neuroblastoma cell lines and on archival specimens of stroma-poor, not MYCN amplified, resectable neuroblastoma tumors, respectively. Results: ALCAM is variously expressed in neuroblastoma cell lines, is shed by metalloproteases and is cleaved by ADAM17/TACE in vitro. ALCAM is expressed in neuroblastoma primary tumors with diverse patterns of subcellular localization and is highly expressed in the neuropil area in a subgroup of cases. Tumor specimens showing high expression of ALCAM at the membrane of the neuroblast body or low levels in the neuropil area are associated with relapse (P=0.044 and P

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalCellular Oncology
Volume32
Issue number1-2
DOIs
Publication statusPublished - 2010

Fingerprint

Activated-Leukocyte Cell Adhesion Molecule
Neuroblastoma
Recurrence
Neoplasms
Neuropil
Cell Line
Sympathetic Nervous System
Metalloproteases
Nervous System
Fluorescent Antibody Technique
Immunohistochemistry
Neoplasm Metastasis
Membranes

Keywords

  • ALCAM/CD166
  • Immunohistochemistry
  • Neuroblastoma
  • Prognosis

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Medicine

Cite this

Different subcellular localization of ALCAM molecules in neuroblastoma : Association with relapse. / Corrias, Maria Valeria; Gambini, Claudio; Gregorio, Andrea; Croce, Michela; Barisione, Gaia; Cossu, Claudia; Rossello, Armando; Ferrini, Silvano; Fabbi, Marina.

In: Cellular Oncology, Vol. 32, No. 1-2, 2010, p. 77-86.

Research output: Contribution to journalArticle

Corrias, MV, Gambini, C, Gregorio, A, Croce, M, Barisione, G, Cossu, C, Rossello, A, Ferrini, S & Fabbi, M 2010, 'Different subcellular localization of ALCAM molecules in neuroblastoma: Association with relapse', Cellular Oncology, vol. 32, no. 1-2, pp. 77-86. https://doi.org/10.3233/CLO-2009-0494
Corrias, Maria Valeria ; Gambini, Claudio ; Gregorio, Andrea ; Croce, Michela ; Barisione, Gaia ; Cossu, Claudia ; Rossello, Armando ; Ferrini, Silvano ; Fabbi, Marina. / Different subcellular localization of ALCAM molecules in neuroblastoma : Association with relapse. In: Cellular Oncology. 2010 ; Vol. 32, No. 1-2. pp. 77-86.
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AU - Gregorio, Andrea

AU - Croce, Michela

AU - Barisione, Gaia

AU - Cossu, Claudia

AU - Rossello, Armando

AU - Ferrini, Silvano

AU - Fabbi, Marina

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AB - Background: The Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166), involved in nervous system development, has been linked to tumor progression and metastasis in several tumors. No information is available on ALCAM expression in neuroblastoma, a childhood neoplasia originating from the sympathetic nervous system. Methods: ALCAM expression was analysed by immunofluorescence and immunohistochemistry on differentiated neuroblastoma cell lines and on archival specimens of stroma-poor, not MYCN amplified, resectable neuroblastoma tumors, respectively. Results: ALCAM is variously expressed in neuroblastoma cell lines, is shed by metalloproteases and is cleaved by ADAM17/TACE in vitro. ALCAM is expressed in neuroblastoma primary tumors with diverse patterns of subcellular localization and is highly expressed in the neuropil area in a subgroup of cases. Tumor specimens showing high expression of ALCAM at the membrane of the neuroblast body or low levels in the neuropil area are associated with relapse (P=0.044 and P

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