Different susceptibility of liver grafts from lean and obese Zucker rats to preservation injury

Andrea Ferrigno, Filippo Carlucci, Antonella Tabucchi, Valentina Tommassini, Vittoria Rizzo, Plinio Richelmi, Enrico Gringeri, Daniele Neri, Eleonora Boncompagni, Isabel Freitas, Umberto Cillo, Mariapia Vairetti

Research output: Contribution to journalArticlepeer-review

Abstract

We compared the susceptibility of liver grafts from lean and obese Zucker rats to preservation injury, using two organ-preservation techniques: conventional static preservation (SP) and machine perfusion (MP) preservation. SP: livers preserved by UW solution at 4, 8 or 20 °C for 6-h. MP: livers perfused for 6-h with an improved oxygenated Krebs-Henseleit solution (KH) at 4, 8 or 20 °C. Reperfusion with KH (2-h) was performed either with the SP or MP preserved livers. Fatty livers tolerate SP poorly at 4, 8 and 20 °C as compared with MP at the same temperatures. SP induced a decrease in the ATP/ADP ratio both at 8 and 20 °C in obese rats while an increase in energy status was found with MP at 8 and 20 °C. Nitrate/nitrite (NOx) concentration was higher and bile flow lower in livers preserved with SP than MP. In lean rats, no differences were observed between MP and SP as regards enzyme release, bile production and NOx levels except for SP at 20 °C in which high enzyme release and low bile flow were observed. In lean rats ATP/ADP was higher and NOx was lower with MP at 20 °C than with SP at 20 °C. To optimize steatotic liver preservation SP should be avoided because it is particularly detrimental as compared with MP.

Original languageEnglish
Pages (from-to)327-334
Number of pages8
JournalCryobiology
Volume59
Issue number3
DOIs
Publication statusPublished - Dec 2009

Keywords

  • Bile
  • Liver
  • Machine perfusion
  • Mitochondria
  • Static preservation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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