Differential action of 3-hydroxyanthranilic acid on viability and activation of stimulated lymphocytes

Elisa Piscianz, Eva Cuzzoni, Sara De Iudicibus, Erica Valencic, Giuliana Decorti, Alberto Tommasini

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Lymphocytes proliferation after antigen-driven activation leads to an increase in cell count, which could last some week, until apoptosis mechanisms allow the homeostatic control of the system. During the first days of this stimulation, activated lymphocytes display high resistance to apoptosis and to most immunosuppressive drugs. According to the literature, few compounds have been described to kill recently activated cells, by inhibiting metabolic processes fundamental to proliferation. The aim of our work was to evaluate comparatively these different compounds, in order to identify the best strategy to kill cells that have undergone proliferation, while sparing the repertoire of resting cells. After preliminary experiments, 3-HAA and bortezomib were selected as the most suitable compounds for our purposes. The possible synergic effect of 3-HAA with bortezomib or with manganese ions was also assessed. 3-HAA was confirmed to be the most reliable pharmacologic approach to inhibit proliferation with acceptable toxicity on resting cells. While in the case of PHA stimulation 3-HAA led to death of most lymphocytes, only a minor percentage of cells were killed after allo-stimulation, suggesting that the effect is proportional to the percentage of stimulated lymphocytes. Manganese ions further enhanced this effect, while results with bortezomib seemed to be less consistent. These results deserve further investigations to develop new procedures for targeting activated cells with pharmacological approaches.

Original languageEnglish
Pages (from-to)2242-2245
Number of pages4
JournalInternational Immunopharmacology
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 2011

Fingerprint

3-Hydroxyanthranilic Acid
Lymphocyte Activation
Lymphocytes
Manganese
Ions
Apoptosis
Immunosuppressive Agents
Cell Count
Pharmacology
Antigens

Keywords

  • 3-hydroxyanthranilic acid
  • Activated lymphocytes
  • Bortezomib
  • Mixed Lymphocyte Reaction
  • Proliferation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Differential action of 3-hydroxyanthranilic acid on viability and activation of stimulated lymphocytes. / Piscianz, Elisa; Cuzzoni, Eva; De Iudicibus, Sara; Valencic, Erica; Decorti, Giuliana; Tommasini, Alberto.

In: International Immunopharmacology, Vol. 11, No. 12, 12.2011, p. 2242-2245.

Research output: Contribution to journalArticle

@article{cd4e783d9e6041c3bc40658c16d0b37d,
title = "Differential action of 3-hydroxyanthranilic acid on viability and activation of stimulated lymphocytes",
abstract = "Lymphocytes proliferation after antigen-driven activation leads to an increase in cell count, which could last some week, until apoptosis mechanisms allow the homeostatic control of the system. During the first days of this stimulation, activated lymphocytes display high resistance to apoptosis and to most immunosuppressive drugs. According to the literature, few compounds have been described to kill recently activated cells, by inhibiting metabolic processes fundamental to proliferation. The aim of our work was to evaluate comparatively these different compounds, in order to identify the best strategy to kill cells that have undergone proliferation, while sparing the repertoire of resting cells. After preliminary experiments, 3-HAA and bortezomib were selected as the most suitable compounds for our purposes. The possible synergic effect of 3-HAA with bortezomib or with manganese ions was also assessed. 3-HAA was confirmed to be the most reliable pharmacologic approach to inhibit proliferation with acceptable toxicity on resting cells. While in the case of PHA stimulation 3-HAA led to death of most lymphocytes, only a minor percentage of cells were killed after allo-stimulation, suggesting that the effect is proportional to the percentage of stimulated lymphocytes. Manganese ions further enhanced this effect, while results with bortezomib seemed to be less consistent. These results deserve further investigations to develop new procedures for targeting activated cells with pharmacological approaches.",
keywords = "3-hydroxyanthranilic acid, Activated lymphocytes, Bortezomib, Mixed Lymphocyte Reaction, Proliferation",
author = "Elisa Piscianz and Eva Cuzzoni and {De Iudicibus}, Sara and Erica Valencic and Giuliana Decorti and Alberto Tommasini",
year = "2011",
month = "12",
doi = "10.1016/j.intimp.2011.09.009",
language = "English",
volume = "11",
pages = "2242--2245",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Differential action of 3-hydroxyanthranilic acid on viability and activation of stimulated lymphocytes

AU - Piscianz, Elisa

AU - Cuzzoni, Eva

AU - De Iudicibus, Sara

AU - Valencic, Erica

AU - Decorti, Giuliana

AU - Tommasini, Alberto

PY - 2011/12

Y1 - 2011/12

N2 - Lymphocytes proliferation after antigen-driven activation leads to an increase in cell count, which could last some week, until apoptosis mechanisms allow the homeostatic control of the system. During the first days of this stimulation, activated lymphocytes display high resistance to apoptosis and to most immunosuppressive drugs. According to the literature, few compounds have been described to kill recently activated cells, by inhibiting metabolic processes fundamental to proliferation. The aim of our work was to evaluate comparatively these different compounds, in order to identify the best strategy to kill cells that have undergone proliferation, while sparing the repertoire of resting cells. After preliminary experiments, 3-HAA and bortezomib were selected as the most suitable compounds for our purposes. The possible synergic effect of 3-HAA with bortezomib or with manganese ions was also assessed. 3-HAA was confirmed to be the most reliable pharmacologic approach to inhibit proliferation with acceptable toxicity on resting cells. While in the case of PHA stimulation 3-HAA led to death of most lymphocytes, only a minor percentage of cells were killed after allo-stimulation, suggesting that the effect is proportional to the percentage of stimulated lymphocytes. Manganese ions further enhanced this effect, while results with bortezomib seemed to be less consistent. These results deserve further investigations to develop new procedures for targeting activated cells with pharmacological approaches.

AB - Lymphocytes proliferation after antigen-driven activation leads to an increase in cell count, which could last some week, until apoptosis mechanisms allow the homeostatic control of the system. During the first days of this stimulation, activated lymphocytes display high resistance to apoptosis and to most immunosuppressive drugs. According to the literature, few compounds have been described to kill recently activated cells, by inhibiting metabolic processes fundamental to proliferation. The aim of our work was to evaluate comparatively these different compounds, in order to identify the best strategy to kill cells that have undergone proliferation, while sparing the repertoire of resting cells. After preliminary experiments, 3-HAA and bortezomib were selected as the most suitable compounds for our purposes. The possible synergic effect of 3-HAA with bortezomib or with manganese ions was also assessed. 3-HAA was confirmed to be the most reliable pharmacologic approach to inhibit proliferation with acceptable toxicity on resting cells. While in the case of PHA stimulation 3-HAA led to death of most lymphocytes, only a minor percentage of cells were killed after allo-stimulation, suggesting that the effect is proportional to the percentage of stimulated lymphocytes. Manganese ions further enhanced this effect, while results with bortezomib seemed to be less consistent. These results deserve further investigations to develop new procedures for targeting activated cells with pharmacological approaches.

KW - 3-hydroxyanthranilic acid

KW - Activated lymphocytes

KW - Bortezomib

KW - Mixed Lymphocyte Reaction

KW - Proliferation

UR - http://www.scopus.com/inward/record.url?scp=81855185551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81855185551&partnerID=8YFLogxK

U2 - 10.1016/j.intimp.2011.09.009

DO - 10.1016/j.intimp.2011.09.009

M3 - Article

C2 - 21979495

AN - SCOPUS:81855185551

VL - 11

SP - 2242

EP - 2245

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 12

ER -