Differential activation of NF-κB subunits in dendritic cells in response to Gram-negative bacteria and to lipopolysaccharide

Susanne Hofer, Maria Rescigno, Francesca Granucci, Stefania Citterio, Maura Francolini, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

Abstract

Dendritic cell (DC) maturation is essential for the initiation of T-dependent immune responses. Nuclear factor kappaB/Rel (NFκB/Rel) transcription factors are ubiquitously expressed signalling molecules, known to regulate the transcription of a large number of genes involved in immune responses, including cytokines such as IL-1, IL-6, TNF-α and cell surface molecules (MHC class I and II, B7.2). In this study, we have compared the activation of five members of the NF-κB family, p65, c-Rel, p50, RelB and p52, during DC maturation in response to lipopolysaccharide (LPS) and to Salmonella typhimurium. We have shown that although the translocation of NF-κB occurred very early, 30 min after treatment with both S. typhimurium and LPS, bacteria-induced NF-κB activation was more pronounced. Four out of five members, i.e. p65, c-Rel, p50 and RelB, were similarly activated upon the two stimuli but with different kinetics. Indeed, we have observed that p65, c-Rel and p50 were translocated early, whereas RelB was translocated later in DC activation. This differential regulation suggests that the various members of NF-κB family can mediate distinct functions of DC physiology.

Original languageEnglish
Pages (from-to)259-265
Number of pages7
JournalMicrobes and Infection
Volume3
Issue number4
DOIs
Publication statusPublished - 2001

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Keywords

  • Dendritic cells
  • Maturation
  • NF-κB
  • Salmonella typhimurium
  • Signal transduction

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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