Abstract
Dendritic cell (DC) maturation is essential for the initiation of T-dependent immune responses. Nuclear factor kappaB/Rel (NFκB/Rel) transcription factors are ubiquitously expressed signalling molecules, known to regulate the transcription of a large number of genes involved in immune responses, including cytokines such as IL-1, IL-6, TNF-α and cell surface molecules (MHC class I and II, B7.2). In this study, we have compared the activation of five members of the NF-κB family, p65, c-Rel, p50, RelB and p52, during DC maturation in response to lipopolysaccharide (LPS) and to Salmonella typhimurium. We have shown that although the translocation of NF-κB occurred very early, 30 min after treatment with both S. typhimurium and LPS, bacteria-induced NF-κB activation was more pronounced. Four out of five members, i.e. p65, c-Rel, p50 and RelB, were similarly activated upon the two stimuli but with different kinetics. Indeed, we have observed that p65, c-Rel and p50 were translocated early, whereas RelB was translocated later in DC activation. This differential regulation suggests that the various members of NF-κB family can mediate distinct functions of DC physiology.
| Original language | English |
|---|---|
| Pages (from-to) | 259-265 |
| Number of pages | 7 |
| Journal | Microbes and Infection |
| Volume | 3 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Dendritic cells
- Maturation
- NF-κB
- Salmonella typhimurium
- Signal transduction
ASJC Scopus subject areas
- Microbiology
- Infectious Diseases
Cite this
Differential activation of NF-κB subunits in dendritic cells in response to Gram-negative bacteria and to lipopolysaccharide. / Hofer, Susanne; Rescigno, Maria; Granucci, Francesca; Citterio, Stefania; Francolini, Maura; Ricciardi-Castagnoli, Paola.
In: Microbes and Infection, Vol. 3, No. 4, 2001, p. 259-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential activation of NF-κB subunits in dendritic cells in response to Gram-negative bacteria and to lipopolysaccharide
AU - Hofer, Susanne
AU - Rescigno, Maria
AU - Granucci, Francesca
AU - Citterio, Stefania
AU - Francolini, Maura
AU - Ricciardi-Castagnoli, Paola
PY - 2001
Y1 - 2001
N2 - Dendritic cell (DC) maturation is essential for the initiation of T-dependent immune responses. Nuclear factor kappaB/Rel (NFκB/Rel) transcription factors are ubiquitously expressed signalling molecules, known to regulate the transcription of a large number of genes involved in immune responses, including cytokines such as IL-1, IL-6, TNF-α and cell surface molecules (MHC class I and II, B7.2). In this study, we have compared the activation of five members of the NF-κB family, p65, c-Rel, p50, RelB and p52, during DC maturation in response to lipopolysaccharide (LPS) and to Salmonella typhimurium. We have shown that although the translocation of NF-κB occurred very early, 30 min after treatment with both S. typhimurium and LPS, bacteria-induced NF-κB activation was more pronounced. Four out of five members, i.e. p65, c-Rel, p50 and RelB, were similarly activated upon the two stimuli but with different kinetics. Indeed, we have observed that p65, c-Rel and p50 were translocated early, whereas RelB was translocated later in DC activation. This differential regulation suggests that the various members of NF-κB family can mediate distinct functions of DC physiology.
AB - Dendritic cell (DC) maturation is essential for the initiation of T-dependent immune responses. Nuclear factor kappaB/Rel (NFκB/Rel) transcription factors are ubiquitously expressed signalling molecules, known to regulate the transcription of a large number of genes involved in immune responses, including cytokines such as IL-1, IL-6, TNF-α and cell surface molecules (MHC class I and II, B7.2). In this study, we have compared the activation of five members of the NF-κB family, p65, c-Rel, p50, RelB and p52, during DC maturation in response to lipopolysaccharide (LPS) and to Salmonella typhimurium. We have shown that although the translocation of NF-κB occurred very early, 30 min after treatment with both S. typhimurium and LPS, bacteria-induced NF-κB activation was more pronounced. Four out of five members, i.e. p65, c-Rel, p50 and RelB, were similarly activated upon the two stimuli but with different kinetics. Indeed, we have observed that p65, c-Rel and p50 were translocated early, whereas RelB was translocated later in DC activation. This differential regulation suggests that the various members of NF-κB family can mediate distinct functions of DC physiology.
KW - Dendritic cells
KW - Maturation
KW - NF-κB
KW - Salmonella typhimurium
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=0035042056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035042056&partnerID=8YFLogxK
U2 - 10.1016/S1286-4579(01)01378-8
DO - 10.1016/S1286-4579(01)01378-8
M3 - Article
C2 - 11334742
AN - SCOPUS:0035042056
VL - 3
SP - 259
EP - 265
JO - Microbes and Infection
JF - Microbes and Infection
SN - 1286-4579
IS - 4
ER -