TY - JOUR
T1 - Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis
AU - D'Aguanno, Simona
AU - Barassi, Alessandra
AU - Lupisella, Santina
AU - d'eril, Gianlodovico Melzi
AU - Del Boccio, Piero
AU - Pieragostino, Damiana
AU - Pallotti, Francesco
AU - Carelli, Valerio
AU - Valentino, Maria Lucia
AU - Liguori, Rocco
AU - Avoni, Patrizia
AU - Bernardini, Sergio
AU - Gambi, Domenico
AU - Urbani, Andrea
AU - Federici, Giorgio
PY - 2008/1
Y1 - 2008/1
N2 - Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.
AB - Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.
KW - Biomarkers
KW - CSF
KW - Leber hereditary optic neuropathy
KW - Multiple sclerosis
KW - Proteomics
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U2 - 10.1016/j.jneuroim.2007.10.004
DO - 10.1016/j.jneuroim.2007.10.004
M3 - Article
C2 - 18061280
AN - SCOPUS:37849041001
VL - 193
SP - 156
EP - 160
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -