Abstract
Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.
| Original language | English |
|---|---|
| Pages (from-to) | 156-160 |
| Number of pages | 5 |
| Journal | Journal of Neuroimmunology |
| Volume | 193 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Jan 2008 |
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Keywords
- Biomarkers
- CSF
- Leber hereditary optic neuropathy
- Multiple sclerosis
- Proteomics
ASJC Scopus subject areas
- Immunology
- Clinical Neurology
- Immunology and Allergy
- Neurology
Cite this
Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis. / D'Aguanno, Simona; Barassi, Alessandra; Lupisella, Santina; d'eril, Gianlodovico Melzi; Del Boccio, Piero; Pieragostino, Damiana; Pallotti, Francesco; Carelli, Valerio; Valentino, Maria Lucia; Liguori, Rocco; Avoni, Patrizia; Bernardini, Sergio; Gambi, Domenico; Urbani, Andrea; Federici, Giorgio.
In: Journal of Neuroimmunology, Vol. 193, No. 1-2, 01.2008, p. 156-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis
AU - D'Aguanno, Simona
AU - Barassi, Alessandra
AU - Lupisella, Santina
AU - d'eril, Gianlodovico Melzi
AU - Del Boccio, Piero
AU - Pieragostino, Damiana
AU - Pallotti, Francesco
AU - Carelli, Valerio
AU - Valentino, Maria Lucia
AU - Liguori, Rocco
AU - Avoni, Patrizia
AU - Bernardini, Sergio
AU - Gambi, Domenico
AU - Urbani, Andrea
AU - Federici, Giorgio
PY - 2008/1
Y1 - 2008/1
N2 - Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.
AB - Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.
KW - Biomarkers
KW - CSF
KW - Leber hereditary optic neuropathy
KW - Multiple sclerosis
KW - Proteomics
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UR - http://www.scopus.com/inward/citedby.url?scp=37849041001&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2007.10.004
DO - 10.1016/j.jneuroim.2007.10.004
M3 - Article
C2 - 18061280
AN - SCOPUS:37849041001
VL - 193
SP - 156
EP - 160
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -