Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis

Simona D'Aguanno; Alessandra Barassi; Santina Lupisella; Gianlodovico Melzi d'eril; Piero Del Boccio; Damiana Pieragostino; Francesco Pallotti; Valerio Carelli; Maria Lucia Valentino; Rocco Liguori; Patrizia Avoni; Sergio Bernardini; Domenico Gambi; Andrea Urbani; Giorgio Federici

doi:10.1016/j.jneuroim.2007.10.004

Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis

Simona D'Aguanno, Alessandra Barassi, Santina Lupisella, Gianlodovico Melzi d'eril, Piero Del Boccio, Damiana Pieragostino, Francesco Pallotti, Valerio Carelli, Maria Lucia Valentino, Rocco Liguori, Patrizia Avoni, Sergio Bernardini, Domenico Gambi, Andrea Urbani, Giorgio Federici

Research output: Contribution to journal › Article › peer-review

Abstract

Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.

Original language	English
Pages (from-to)	156-160
Number of pages	5
Journal	Journal of Neuroimmunology
Volume	193
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2007.10.004
Publication status	Published - Jan 2008

Keywords

Biomarkers
CSF
Leber hereditary optic neuropathy
Multiple sclerosis
Proteomics

ASJC Scopus subject areas

Immunology
Clinical Neurology
Immunology and Allergy
Neurology

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D'Aguanno, S., Barassi, A., Lupisella, S., d'eril, G. M., Del Boccio, P., Pieragostino, D., Pallotti, F., Carelli, V., Valentino, M. L., Liguori, R., Avoni, P., Bernardini, S., Gambi, D., Urbani, A., & Federici, G. (2008). Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis. Journal of Neuroimmunology, 193(1-2), 156-160. https://doi.org/10.1016/j.jneuroim.2007.10.004

Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis. / D'Aguanno, Simona; Barassi, Alessandra; Lupisella, Santina; d'eril, Gianlodovico Melzi; Del Boccio, Piero; Pieragostino, Damiana; Pallotti, Francesco; Carelli, Valerio ; Valentino, Maria Lucia ; Liguori, Rocco ; Avoni, Patrizia; Bernardini, Sergio; Gambi, Domenico; Urbani, Andrea; Federici, Giorgio.

In: Journal of Neuroimmunology, Vol. 193, No. 1-2, 01.2008, p. 156-160.

Research output: Contribution to journal › Article › peer-review

D'Aguanno, S, Barassi, A, Lupisella, S, d'eril, GM, Del Boccio, P, Pieragostino, D, Pallotti, F, Carelli, V , Valentino, ML , Liguori, R , Avoni, P, Bernardini, S, Gambi, D, Urbani, A & Federici, G 2008, 'Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis', Journal of Neuroimmunology, vol. 193, no. 1-2, pp. 156-160. https://doi.org/10.1016/j.jneuroim.2007.10.004

D'Aguanno, Simona ; Barassi, Alessandra ; Lupisella, Santina ; d'eril, Gianlodovico Melzi ; Del Boccio, Piero ; Pieragostino, Damiana ; Pallotti, Francesco ; Carelli, Valerio ; Valentino, Maria Lucia ; Liguori, Rocco ; Avoni, Patrizia ; Bernardini, Sergio ; Gambi, Domenico ; Urbani, Andrea ; Federici, Giorgio. / Differential cerebro spinal fluid proteome investigation of Leber hereditary optic neuropathy (LHON) and multiple sclerosis. In: Journal of Neuroimmunology. 2008 ; Vol. 193, No. 1-2. pp. 156-160.

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abstract = "Leber's hereditary optic neuropathy (LHON) is a genetic disease leading to the loss of central vision and optic nerve atrophy. The existence of occasional cases of LHON patients developing a Multiple Sclerosis (MS)-like illness and the hypothesis that mtDNA variants may be involved in MS suggest the possibility of some common molecular mechanisms linking the two diseases. We have pursued a comparative proteomics approach on cerebrospinal fluid (CSF) samples from LHON and MS patients, as well as healthy donors by employing 2-DE gel separations coupled to MALDI-TOF-MS and nLC-MS/MS investigations. 7 protein spots showed significant differential distribution among the three groups. Both CSF of LHON or MS patients are characterized by lower level of transthyretin dimer adduct while a specific up regulation of Apo A-IV was detected in LHON CSF.",

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AU - Pieragostino, Damiana

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AU - Carelli, Valerio

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AU - Liguori, Rocco

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