Differential cytogenomics and miRNA signature of the acute myeloid leukaemia Kasumi-1 cell line CD34+38- compartment

Laura Pedranzini, Federica Mottadelli, Simona Ronzoni, Franca Rossella, Manuela Ferracin, Ivana Magnani, Gaia Roversi, Patrizia Colapietro, Massimo Negrini, Pier Giuseppe Pelicci, Lidia Larizza

Research output: Contribution to journalArticlepeer-review

Abstract

The t(8;21) Acute Myeloid Leukaemia (AML) Kasumi-1 cell line with N822K KIT mutation, is a model system for leukemogenesis. As AML initiating cells reside in the CD34+CD38- fraction, we addressed the refined cytogenomic characterization and miRNA expression of Kasumi-1 cell line and its FACS-sorted subpopulations focussing on this compartment. By conventional cytogenetics, Spectral-Karyotyping and array-CGH the cytogenomic profile of Kasumi-1 cells evidenced only subtle regions differentially represented in CD34+CD38- cells. Expression profiling by a miRNA platform showed a set of miRNA differentially expressed in paired subpopulations and the signature of miR-584 and miR-182 upregulation in the CD34+CD38- fraction.

Original languageEnglish
Pages (from-to)1287-1295
Number of pages9
JournalLeukemia Research
Volume34
Issue number10
DOIs
Publication statusPublished - Oct 2010

Keywords

  • AML
  • CD34CD38
  • Cytogenomic characterization
  • Kasumi-1
  • MiRNA expression

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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