Differential Denaturation of Serum Proteome Reveals a Significant Amount of Hidden Information in Complex Mixtures of Proteins

Vincenzo Verdoliva, Cinzia Senatore, Maria Letizia Polci, Stefania Rossi, Martina Cordella, Giuseppe Carlucci, Paolo Marchetti, Giancarlo Antonini-Cappellini, Antonio Facchiano, Daniela D'Arcangelo, Francesco Facchiano

Research output: Contribution to journalArticle

Abstract

Recently developed proteomic technologies allow to profile thousands of proteins within a high-throughput approach towards biomarker discovery, although results are not as satisfactory as expected. In the present study we demonstrate that serum proteome denaturation is a key underestimated feature; in fact, a new differential denaturation protocol better discriminates serum proteins according to their electrophoretic mobility as compared to single-denaturation protocols. Sixty nine different denaturation treatments were tested and the 3 most discriminating ones were selected (TRIDENT analysis) and applied to human sera, showing a significant improvement of serum protein discrimination as confirmed by MALDI-TOF/MS and LC-MS/MS identification, depending on the type of denaturation applied. Thereafter sera from mice and patients carrying cutaneous melanoma were analyzed through TRIDENT. Nine and 8 protein bands were found differentially expressed in mice and human melanoma sera, compared to healthy controls (p

Original languageEnglish
Article numbere57104
JournalPLoS One
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 22 2013

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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