Differential distribution of hepatitis B core and E antigens in hepatocytes

Analysis by monoclonal antibodies

M. Mondelli, R. S. Tedder, B. Ferns, P. Pontisso, G. Realdi, A. Alberti

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Current knowledge on the expression of HBeAg in hepatocytes is incomplete because of difficulties in obtaining monospecific antisera devoid of anti-HBc reactivity. In this study, we have examined by immunofluorescence the expression of HBcAg and HBeAg in cryostat liver sections from 25 chronic carriers of HBsAg using monoclonal antibodies. Although virtually all liver biopsies displayed concordance for HBeAg and HBcAg expression, the pattern of fluorescence differed markedly. Thus, monoclonal anti-HBc gave nuclear staining in all 13 reactive biopsies, while cytoplasmic staining was observed in only two of these. In contrast, monoclonal anti-HBe showed cytoplasmic reactivity coexisting with nuclear reactivity in 10 of 13 reactive biopsies. Hepatitis B virus DNA polymerase activity in the serum appeared to correlate better with the presence of HBcAg in hepatocytes rather than HBeAg. These results provide further evidence that HBeAg is expressed both in the nuclei and in the cytoplasm of infected hepatocytes. The observation that the number of cells expressing HBeAg exceeds those expressing HBcAg in carriers with active virus replication would suggest that assembly of core particles occurs in only a proportion of infected hepatocytes expressing HBeAg.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
JournalHepatology
Volume6
Issue number2
Publication statusPublished - 1986

Fingerprint

Hepatitis E
Hepatitis B Core Antigens
Hepatitis B e Antigens
Hepatocytes
Monoclonal Antibodies
Biopsy
Staining and Labeling
Liver
DNA-Directed DNA Polymerase
Hepatitis B Surface Antigens
Virus Replication
Hepatitis B virus
Fluorescent Antibody Technique
Immune Sera
Cytoplasm
Cell Count
Fluorescence
Serum

ASJC Scopus subject areas

  • Hepatology

Cite this

Mondelli, M., Tedder, R. S., Ferns, B., Pontisso, P., Realdi, G., & Alberti, A. (1986). Differential distribution of hepatitis B core and E antigens in hepatocytes: Analysis by monoclonal antibodies. Hepatology, 6(2), 199-204.

Differential distribution of hepatitis B core and E antigens in hepatocytes : Analysis by monoclonal antibodies. / Mondelli, M.; Tedder, R. S.; Ferns, B.; Pontisso, P.; Realdi, G.; Alberti, A.

In: Hepatology, Vol. 6, No. 2, 1986, p. 199-204.

Research output: Contribution to journalArticle

Mondelli, M, Tedder, RS, Ferns, B, Pontisso, P, Realdi, G & Alberti, A 1986, 'Differential distribution of hepatitis B core and E antigens in hepatocytes: Analysis by monoclonal antibodies', Hepatology, vol. 6, no. 2, pp. 199-204.
Mondelli, M. ; Tedder, R. S. ; Ferns, B. ; Pontisso, P. ; Realdi, G. ; Alberti, A. / Differential distribution of hepatitis B core and E antigens in hepatocytes : Analysis by monoclonal antibodies. In: Hepatology. 1986 ; Vol. 6, No. 2. pp. 199-204.
@article{0491969cf2ed44f2a12f19fe316211cf,
title = "Differential distribution of hepatitis B core and E antigens in hepatocytes: Analysis by monoclonal antibodies",
abstract = "Current knowledge on the expression of HBeAg in hepatocytes is incomplete because of difficulties in obtaining monospecific antisera devoid of anti-HBc reactivity. In this study, we have examined by immunofluorescence the expression of HBcAg and HBeAg in cryostat liver sections from 25 chronic carriers of HBsAg using monoclonal antibodies. Although virtually all liver biopsies displayed concordance for HBeAg and HBcAg expression, the pattern of fluorescence differed markedly. Thus, monoclonal anti-HBc gave nuclear staining in all 13 reactive biopsies, while cytoplasmic staining was observed in only two of these. In contrast, monoclonal anti-HBe showed cytoplasmic reactivity coexisting with nuclear reactivity in 10 of 13 reactive biopsies. Hepatitis B virus DNA polymerase activity in the serum appeared to correlate better with the presence of HBcAg in hepatocytes rather than HBeAg. These results provide further evidence that HBeAg is expressed both in the nuclei and in the cytoplasm of infected hepatocytes. The observation that the number of cells expressing HBeAg exceeds those expressing HBcAg in carriers with active virus replication would suggest that assembly of core particles occurs in only a proportion of infected hepatocytes expressing HBeAg.",
author = "M. Mondelli and Tedder, {R. S.} and B. Ferns and P. Pontisso and G. Realdi and A. Alberti",
year = "1986",
language = "English",
volume = "6",
pages = "199--204",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Differential distribution of hepatitis B core and E antigens in hepatocytes

T2 - Analysis by monoclonal antibodies

AU - Mondelli, M.

AU - Tedder, R. S.

AU - Ferns, B.

AU - Pontisso, P.

AU - Realdi, G.

AU - Alberti, A.

PY - 1986

Y1 - 1986

N2 - Current knowledge on the expression of HBeAg in hepatocytes is incomplete because of difficulties in obtaining monospecific antisera devoid of anti-HBc reactivity. In this study, we have examined by immunofluorescence the expression of HBcAg and HBeAg in cryostat liver sections from 25 chronic carriers of HBsAg using monoclonal antibodies. Although virtually all liver biopsies displayed concordance for HBeAg and HBcAg expression, the pattern of fluorescence differed markedly. Thus, monoclonal anti-HBc gave nuclear staining in all 13 reactive biopsies, while cytoplasmic staining was observed in only two of these. In contrast, monoclonal anti-HBe showed cytoplasmic reactivity coexisting with nuclear reactivity in 10 of 13 reactive biopsies. Hepatitis B virus DNA polymerase activity in the serum appeared to correlate better with the presence of HBcAg in hepatocytes rather than HBeAg. These results provide further evidence that HBeAg is expressed both in the nuclei and in the cytoplasm of infected hepatocytes. The observation that the number of cells expressing HBeAg exceeds those expressing HBcAg in carriers with active virus replication would suggest that assembly of core particles occurs in only a proportion of infected hepatocytes expressing HBeAg.

AB - Current knowledge on the expression of HBeAg in hepatocytes is incomplete because of difficulties in obtaining monospecific antisera devoid of anti-HBc reactivity. In this study, we have examined by immunofluorescence the expression of HBcAg and HBeAg in cryostat liver sections from 25 chronic carriers of HBsAg using monoclonal antibodies. Although virtually all liver biopsies displayed concordance for HBeAg and HBcAg expression, the pattern of fluorescence differed markedly. Thus, monoclonal anti-HBc gave nuclear staining in all 13 reactive biopsies, while cytoplasmic staining was observed in only two of these. In contrast, monoclonal anti-HBe showed cytoplasmic reactivity coexisting with nuclear reactivity in 10 of 13 reactive biopsies. Hepatitis B virus DNA polymerase activity in the serum appeared to correlate better with the presence of HBcAg in hepatocytes rather than HBeAg. These results provide further evidence that HBeAg is expressed both in the nuclei and in the cytoplasm of infected hepatocytes. The observation that the number of cells expressing HBeAg exceeds those expressing HBcAg in carriers with active virus replication would suggest that assembly of core particles occurs in only a proportion of infected hepatocytes expressing HBeAg.

UR - http://www.scopus.com/inward/record.url?scp=0022628279&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022628279&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 199

EP - 204

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 2

ER -