Differential effect of α-latrotoxin on exocytosis from small synaptic vesicles and from large dense-core vesicles containing calcitonin gene-related peptide at the frog neuromuscular junction

M. Matteoli, C. Haimann, F. Torri-Tarelli, J. M. Polak, B. Ceccarelli, P. De Camilli

Research output: Contribution to journalArticlepeer-review

Abstract

The regulatory peptide called calcitonin gene-related peptide (CGRP) was detected by immunofluorescence in frog motor neurons and motor nerve terminals. In motor nerve terminals, CGRP-like immunoreactivity was found to be segregated within large dense-core vesicles. To determine whether exocytosis from acetylcholine-containing small synaptic vesicles and from CGRP-containing large dense-core vesicles can be independently stimulated, nerve-muscle preparations were exposed to α-latrotoxin. This toxin induced complete depletion of acetylcholine-containing small synaptic vesicles but did not induce a parallel depletion of CGRP-like immunoreactivity and of large dense-core vesicles. These effects were independent of the presence of extracellular Ca2+ and occurred both at room temperature and at low temperature (1-3°C). These findings suggest that exocytosis from the two vesicle populations is mediated by distinct biochemical mechanisms, which might be differentially regulated by physiological stimuli.

Original languageEnglish
Pages (from-to)7366-7370
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number19
Publication statusPublished - 1988

ASJC Scopus subject areas

  • General
  • Genetics

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