Differential effect of 9β-chloro-16,16-dimethyl prostaglandin E2 (nocloprost) on the radiation response of human normal fibroblasts and colon adenocarcinoma cells

N. Zaffaroni, R. Villa, L. Orlandi, A. De Pascale, S. Del Mastro, R. Silvestrini

Research output: Contribution to journalArticle

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Abstract

The possible influence of 9β-chloro-16,16-dimethyl prostaglandin E2 (nocloprost) on the effect of 137Cs γ irradiation was investigated comparatively in human normal fibroblasts and colon adenocarcinoma cells. By itself, the compound did not influence the proliferation of cells of either cell type or the clonogenic capacity of carcinoma cells. Moreover, nocloprost did not induce any DNA strand breakage, as evaluated by neutral elution, in cells of either cell type. A 2-h incubation with nocloprost before irradiation induced an enhancement of fibroblast survival after an exposure of 10 Gy. This protective effect was not observed in adenocarcinoma cells under the same treatment conditions. The amount of double-strand breaks induced by 50 Gy was reduced in normal cells but not in tumor cells exposed to the prostaglandin analog before irradiation. Moreover, incubation with nocloprost for 2 h after irradiation remarkably enhanced the rate of rejoining of DNA breaks in fibroblasts but not in adenocarcinoma cells. Overall, these findings indicate a specific radioprotective effect of nocloprost in normal cells and no influence of the compound on the cytotoxic effect of ionizing radiation on colon adenocarcinoma cells.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalRadiation Research
Volume135
Issue number1
Publication statusPublished - 1993

Fingerprint

prostaglandins
fibroblasts
adenocarcinoma
Dinoprostone
colon
Colon
Adenocarcinoma
Fibroblasts
Radiation
radiation
cells
irradiation
strands
deoxyribonucleic acid
nocloprost
synthetic prostaglandins
Synthetic Prostaglandins
elution
DNA Breaks
DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Differential effect of 9β-chloro-16,16-dimethyl prostaglandin E2 (nocloprost) on the radiation response of human normal fibroblasts and colon adenocarcinoma cells. / Zaffaroni, N.; Villa, R.; Orlandi, L.; De Pascale, A.; Del Mastro, S.; Silvestrini, R.

In: Radiation Research, Vol. 135, No. 1, 1993, p. 88-92.

Research output: Contribution to journalArticle

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AU - De Pascale, A.

AU - Del Mastro, S.

AU - Silvestrini, R.

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