Differential effects of aspirin and indomethacin on platelet and leukocyte thromboxane A2 formation

Paola Maderna, Susanna Colli, Gabriele Morazzoni, Eduardo Stragliotto, Elena Tremoli

Research output: Contribution to journalArticle

Abstract

In this study, the in vitro inhibitory effects of aspirin and indomethacin on the arachidonic acid induced thromboxane B2 formation by human leukocytes, are evaluated. The results are compared to those obtained using similarly challenged human washed platelets. Acetylsalicylic acid inhibition, calculated as IC50 by a dose-response curve, is more than ten fold higher for leukocytes vs platelets. In fact, a concentration of aspirin as low as 5 μM almost completely suppresses thromboxane B2 formation by washed platelets, whereas a concentration o of 50 μM is necessary to achieve the same inhibition with leukocytes. Indomethacin (0.001-100 μM), incubated with platelet and leukocyte suspensions acts similarly to aspirin. Leukocyte and platelet cyclooxygenases are, therefore, differently affected by both aspirin and indomethacin. These differences may be relevant in the understanding of the wide divergence between the aspirin doses used in thrombosis prevention and in the treatment of inflammatory disease.

Original languageEnglish
Pages (from-to)379-391
Number of pages13
JournalProstaglandins, Leukotrienes and Medicine
Volume18
Issue number3
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

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