Differential effects of CGRP on adenylyl cyclase in adult and embryonic rat brain

Isabella Villa, Anna Maria Colangelo, Angelo L. Vescovi, Angela Gritti, Sergio Schinelli, Francesca Pagani, Francesca Guidobono

Research output: Contribution to journalArticlepeer-review

Abstract

The presence of functional receptors for calcitonin gene- related peptide (CGRP) in the brain of adult rats and on nerve cell cultures was investigated. Neuronal and glial cultures were obtained from mesencephalons of embryos at gestational day 16. The response to CGRP was tested by measuring the adenylyl cyclase (AC) activity on isolated membranes. CGRP binding in adult rat brains was ineffective in activating AC, whereas a dose-dependent stimulation of AC activity was induced by the peptide both in neuronal and glial cultures. This effect was more pronounced in the glial cells where high affinity binding sites for CGRP were detected. The presence of functional CGRP receptors in embryonic mesencephalic cells, suggests a role for CGRP in the development of rat mesencephalon.

Original languageEnglish
Pages (from-to)2489-2492
Number of pages4
JournalNeuroReport
Volume5
Issue number18
Publication statusPublished - 1994

Keywords

  • Adenylyl cyclase activity
  • Binding
  • CGRP
  • Glial cell cultures

ASJC Scopus subject areas

  • Neuroscience(all)

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