Differential effects of distamycin analogues on amplification of human gene sequences by polymerase-chain reaction

M. Passadore, N. Bianchi, G. Feriotto, C. Mischiati, P. Giacomini, R. Piva, R. Gambari

Research output: Contribution to journalArticle

Abstract

In this report we analyse the effects of distamycin and five distamycin analogues on amplification by polymerase-chain reaction (PCR) of two gene sequences displaying a different A+T/G+C content. The first was a 5' region of the human oestrogen receptor (ER) gene, containing a (TA)26 stretch; the second was a CG-rich sequence of the human Ha-ras oncogene. The results obtained unequivocally demonstrate that the addition of one pyrrole ring significantly improves the ability of distamycin derivatives to interfere with PCR-mediated amplification of the human ER genomic region carrying a (TA)26 stretch. The distamycin analogues analysed differ in the number of pyrrole rings and in the presence of an N-formyl, an N-formimidoyl or a retroamide group at position X1. Among compounds carrying the same number of pyrrole rings, those carrying an N-formyl or an N-formimidoyl group retain a similar inhibitory activity. The retroamide analogues, on the contrary, are much less efficient in inhibiting PCR-mediated amplification of the 5' ER region. With respect to sequence selectivity both distamycin and distamycin analogues exhibit a sequence preference, since they do not inhibit PCR amplification of Ha-ras CG-rich gene regions, with the exception of a distamycin analogue carrying four pyrrole rings.

Original languageEnglish
Pages (from-to)513-519
Number of pages7
JournalBiochemical Journal
Volume308
Issue number2
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Biochemistry

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    Passadore, M., Bianchi, N., Feriotto, G., Mischiati, C., Giacomini, P., Piva, R., & Gambari, R. (1995). Differential effects of distamycin analogues on amplification of human gene sequences by polymerase-chain reaction. Biochemical Journal, 308(2), 513-519.