TY - JOUR
T1 - Differential effects of retinoic acid isomers on the expression of nuclear receptor co-regulators in neuroblastoma
AU - Lovat, Penny E.
AU - Annicchiarico-Petruzzelli, Margherita
AU - Corazzari, Marco
AU - Dobson, Mark G.
AU - Malcolm, Archie J.
AU - Pearson, Andy D J
AU - Melino, Gerry
AU - Redfern, Christopher P F
PY - 1999/2/26
Y1 - 1999/2/26
N2 - Retinoic acid modulates growth and induces differentiation and apoptosis of neuroblastoma cells in vitro, with the all-trans and 9-cis isomers having different biological properties. Transcriptional activation in response to retinoic acid isomers is mediated by retinoic acid receptors and retinoid X receptors. The differential expression of co-activators and co-repressors which preferentially interact with retinoic acid receptors or retinoid X receptors may be a mechanism leading to different cellular responses to 9-cis and all-trans retinoic acid. To test this hypothesis, we have studied the expression of the nuclear receptor co-regulators TIF1α, TIF1β, SUG1 and SMRT in the N-type and S-type neuroblastoma cell lines SH SY 5Y and SH S EP. Transcripts for all four co-regulators were expressed in these neuroblastoma cells. The expression of TIF1α, TIF1β and SUG1 did not change in response to retinoic acid; however, SMRT was induced in both neuroblastoma cell lines, but particularly by all-trans retinoic acid in SH S EP cells. An additional co-activator, Trip3, was isolated by differential mRNA display and shown to be preferentially induced by 9-cis retinoic acid in SH SY 5Y and SH S EP cells. These data suggest that retinoic acid isomer-specific induction of nuclear receptor co-regulators may determine, in part, the differential biological effects of retinoic acid isomers. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - Retinoic acid modulates growth and induces differentiation and apoptosis of neuroblastoma cells in vitro, with the all-trans and 9-cis isomers having different biological properties. Transcriptional activation in response to retinoic acid isomers is mediated by retinoic acid receptors and retinoid X receptors. The differential expression of co-activators and co-repressors which preferentially interact with retinoic acid receptors or retinoid X receptors may be a mechanism leading to different cellular responses to 9-cis and all-trans retinoic acid. To test this hypothesis, we have studied the expression of the nuclear receptor co-regulators TIF1α, TIF1β, SUG1 and SMRT in the N-type and S-type neuroblastoma cell lines SH SY 5Y and SH S EP. Transcripts for all four co-regulators were expressed in these neuroblastoma cells. The expression of TIF1α, TIF1β and SUG1 did not change in response to retinoic acid; however, SMRT was induced in both neuroblastoma cell lines, but particularly by all-trans retinoic acid in SH S EP cells. An additional co-activator, Trip3, was isolated by differential mRNA display and shown to be preferentially induced by 9-cis retinoic acid in SH SY 5Y and SH S EP cells. These data suggest that retinoic acid isomer-specific induction of nuclear receptor co-regulators may determine, in part, the differential biological effects of retinoic acid isomers. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - 9-cis retinoic acid
KW - All-trans retinoic acid
KW - Co-activator
KW - Co-repressor
KW - Neuroblastoma
KW - SMRT
KW - TIF1
KW - Trip3
UR - http://www.scopus.com/inward/record.url?scp=0032979843&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032979843&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(99)00162-3
DO - 10.1016/S0014-5793(99)00162-3
M3 - Article
C2 - 10094499
AN - SCOPUS:0032979843
VL - 445
SP - 415
EP - 419
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 2-3
ER -